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Quantitative Methods in Proteomics

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  • © 2021

Overview

Editors:
  1. Katrin Marcus

    1. Medizinisches Proteom-Center (MPC), Medical Faculty, Ruhr-Universität Bochum, Bochum, Germany

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  2. Martin Eisenacher

    1. Medical Proteome Analysis, Center for Proteindiagnostics (PRODI), Ruhr-University Bochum, Bochum, Germany

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    You can also search for this editor in PubMed   Google Scholar

  3. Barbara Sitek

    1. Klinik für Anästhesiologie, Intensivmedizin und Schmerztherapie, Universitätsklinikum Knappschaftskrankenhaus Bochum, Bochum, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2228)

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Table of contents (31 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Important Issues in Planning a Proteomics Experiment : Statistical Considerations of Quantitative Proteomic Data

    • Karin Schork, Katharina Podwojski, Michael Turewicz, Christian Stephan, Martin Eisenacher
    Pages 1-20Open Access

  3. Good Old-Fashioned Protein Concentration Determination by Amino Acid Analysis

    • Caroline May, Bettina Serschnitzki, Katrin Marcus
    Pages 21-28

  4. Protein Quantification Using the “Rapid Western Blot” Approach

    • Katalin Barkovits, Kathy Pfeiffer, Britta Eggers, Katrin Marcus
    Pages 29-39

  5. The Whereabouts of 2D Gels in Quantitative Proteomics

    • Thierry Rabilloud, Cécile Lelong
    Pages 41-51

  6. Proteome Analysis with Classical 2D-PAGE

    • Caroline May, Frederic Brosseron, Kathy Pfeiffer, Kristin Fuchs, Helmut E. Meyer, Barbara Sitek et al.
    Pages 53-62

  7. Silver Staining of 2D Electrophoresis Gels

    • Thierry Rabilloud
    Pages 63-75

  8. Differential Proteome Analysis Using 2D-DIGE

    • Caroline May, Frederic Brosseron, Piotr Chartowski, Kristin Fuchs, Helmut E. Meyer, Barbara Sitek et al.
    Pages 77-84

  9. Quantitative Mass Spectrometry-Based Proteomics: An Overview

    • Svitlana Rozanova, Katalin Barkovits, Miroslav Nikolov, Carla Schmidt, Henning Urlaub, Katrin Marcus
    Pages 85-116Open Access

  10. Tandem Mass Tags for Comparative and Discovery Proteomics

    • Oliver Pagel, Laxmikanth Kollipara, Albert Sickmann
    Pages 117-131

  11. An Approach for Triplex-IPTL

    • Christian J. Koehler, Bernd Thiede
    Pages 133-144

  12. Targeted Protein Quantification Using Parallel Reaction Monitoring (PRM)

    • Katalin Barkovits, Weiqiang Chen, Michael Kohl, Thilo Bracht
    Pages 145-157

  13. Quantitative Approach Using Matrix-Assisted Laser Desorption/ Ionization Time-of-Flight (MALDI-ToF) Mass Spectrometry

    • Brooke A. Dilmetz, Peter Hoffmann, Mark R. Condina
    Pages 159-166

  14. Application of SILAC Labeling in Phosphoproteomics Analysis

    • Markus Stepath, Thilo Bracht
    Pages 167-183

  15. Relative Quantification of Phosphorylated and Glycosylated Peptides from the Same Sample Using Isobaric Chemical Labelling with a Two-Step Enrichment Strategy

    • Ivan Silbern, Pan Fang, Yanlong Ji, Lenz Christof, Henning Urlaub, Kuan-Ting Pan
    Pages 185-203

  16. High-Throughput Profiling of Proteome and Posttranslational Modifications by 16-Plex TMT Labeling and Mass Spectrometry

    • Kaiwen Yu, Zhen Wang, Zhiping Wu, Haiyan Tan, Ashutosh Mishra, Junmin Peng
    Pages 205-224

  17. Quantification and Identification of Post-Translational Modifications Using Modern Proteomics Approaches

    • Anja Holtz, Nathan Basisty, Birgit Schilling
    Pages 225-235Open Access

  18. Affinity Enrichment Chemoproteomics for Target Deconvolution and Selectivity Profiling

    • Thilo Werner, Michael Steidel, H. Christian Eberl, Marcus Bantscheff
    Pages 237-252

  19. 2nSILAC for Quantitative Proteomics of Prototrophic Baker’s Yeast

    • Stefan Dannenmaier, Silke Oeljeklaus, Bettina Warscheid
    Pages 253-270Open Access

  20. Metabolic Labeling of Clostridioides difficile Proteins

    • Anke Trautwein-Schult, Jürgen Bartel, Sandra Maaß, Dörte Becher
    Pages 271-282
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Keywords

About this book

This second edition provides new and updated methods on the principles underlying modern protein analysis, from statistical issues to gel-based and mass spectrometry-based applications. Chapters detail protein quantification as basis for realisation of quantitative studies, gel-based and mass spectrometry-based quantification techniques, TMT, IPTL, PRM, MALDI Imaging, SILAC, PTM analysis, DIA, cross-linking, and the up-to-date topics of software and data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge,  Quantitative Methods in Proteomics, Second Edition aims to provide comprehensive and competent overview in the important and still growing field of quantitative proteomics.

Editors and Affiliations

  • Medizinisches Proteom-Center (MPC), Medical Faculty, Ruhr-Universität Bochum, Bochum, Germany

    Katrin Marcus

  • Medical Proteome Analysis, Center for Proteindiagnostics (PRODI), Ruhr-University Bochum, Bochum, Germany

    Martin Eisenacher

  • Klinik für Anästhesiologie, Intensivmedizin und Schmerztherapie, Universitätsklinikum Knappschaftskrankenhaus Bochum, Bochum, Germany

    Barbara Sitek

Bibliographic Information

  • Book Title: Quantitative Methods in Proteomics

  • Editors: Katrin Marcus, Martin Eisenacher, Barbara Sitek

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1024-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1023-7Published: 06 May 2021

  • Softcover ISBN: 978-1-0716-1026-8Published: 07 May 2022

  • eBook ISBN: 978-1-0716-1024-4Published: 05 May 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XV, 483

  • Number of Illustrations: 20 b/w illustrations, 65 illustrations in colour

  • Topics: Proteomics

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