Listeria Monocytogenes

1. Front Matter

   Pages i-xi

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2. Detection, Quantification, and Confirmation

   1. Front Matter

      Pages 1-1

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   2. Traditional Methods of Analysis for Listeria monocytogenes

      • Rui Magalhães, Cristina Mena, Vânia Ferreira, Gonçalo Almeida, Joana Silva, Paula Teixeira

      Pages 3-16

   3. MALDI-ToF MS: A Rapid Methodology for Identifying and Subtyping Listeria monocytogenes

      • Snehal R. Jadhav, Rohan M. Shah, Enzo A. Palombo

      Pages 17-29

   4. Sample Preparation for qPCR Detection of Listeria from Food

      • Patrick Mester, Anna Kristina Witte, Peter Rossmanith

      Pages 31-40

   5. qPCR Validation on the Basis of the Listeria monocytogenes prfA Assay

      • Anna Kristina Witte, Patrick Mester, Peter Rossmanith

      Pages 41-53

3. Subtyping Approaches

   1. Front Matter

      Pages 55-55

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   2. Serotype Assignment by Sero-agglutination, ELISA, and PCR

      • Lisa Gorski

      Pages 57-78

   3. Pulsed-Field Gel Electrophoresis (PFGE) Analysis of Listeria monocytogenes

      • Karen Hunt, Kieran Jordan

      Pages 79-88

   4. Multilocus Sequence Typing (MLST) and Whole Genome Sequencing (WGS) of Listeria monocytogenes and Listeria innocua

      • Beatrix Stessl, Martin Wagner, Werner Ruppitsch

      Pages 89-103

4. Genotypic and Phenotypic Characterization

   1. Front Matter

      Pages 105-105

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   2. High-Throughput Characterization of Listeria monocytogenes Using the OmniLog Phenotypic Microarray

      • Laura Luque-Sastre, Kieran Jordan, Séamus Fanning, Edward M. Fox

      Pages 107-113

   3. High-Throughput Screening of Biofilm Formation of Listeria monocytogenes on Stainless Steel Coupons Using a 96-Well Plate Format

      • Jessica A. Gray, P. Scott Chandry, John P. Bowman, Edward M. Fox

      Pages 115-122

   4. Confocal Laser Microscopy Analysis of Listeria monocytogenes Biofilms and Spatially Organized Communities

      • Maud Darsonval, Marina Grégoire, Julien Deschamps, Romain Briandet

      Pages 123-136

   5. Extraction and Preparation of Listeria monocytogenes Subproteomes for Mass Spectrometry Analysis

      • Tiago Santos, Michel Hébraud

      Pages 137-153

5. Strain Manipulation

   1. Front Matter

      Pages 155-155

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   2. Extraction and Analysis of Plasmid DNA from Listeria monocytogenes

      • Aidan Casey, Olivia McAuliffe

      Pages 157-163

   3. Generation of Nonpolar Deletion Mutants in Listeria monocytogenes Using the “SOEing” Method

      • Kathrin Rychli, Eva Wagner, Caitriona M. Guinane, Karen Daly, Colin Hill, Paul D. Cotter

      Pages 165-175

   4. Mutant Construction and Integration Vector-Mediated Genetic Complementation in Listeria monocytogenes

      • Cameron Parsons, Reha Azizoglu, Driss Elhanafi, Sophia Kathariou

      Pages 177-185

6. Host-Pathogen Interactions

   1. Front Matter

      Pages 187-187

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This updated book explores a wide repertoire of tools and approaches that have been created, modified, and applied to the study of L. monocytogenes, forming the basis of our understanding of the bacterium today. Many of these key experimental techniques are gathered together herein. The volume presents aspects such as clinical disease and host-pathogen interactions, as well as the study of biofilms which present a significant challenge for control of the organism in the food processing environment. The topics covered in this edition also include sampling in order to isolate Listeria, methods for their identification and characterization, methods for gene manipulation, and methods for control of the organism. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshootingand avoiding known pitfalls. 

Authoritative and up-to-date, Listeria monocytogenes: Methods and Protocols, Second Edition aims to contribute toward the harmonization of methods used to study this important bacterium, and to be of particular interest to Listeria research both in relation to food association and control as well as clinical microbiology.

• Bacterium
• Foodborne transmission
• Public health
• Biofilm formation
• Food production chains
• Host-pathogen interactions
• Clinical microbiology

• Department of Applied Sciences, Northumbria University, Newcastle-upon-Tyne, UK

  Edward M. Fox

• INRAE, AgroParisTech, Micalis Institute,, Université Paris-Saclay, Jouy-en-Josas, France

  Hélène Bierne

• Institute of Food Safety Food Technology and Veterinary Public Health, University of Veterinary Medicine Vienna, Vienna, Austria

  Beatrix Stessl

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