Skip to main content
SpringerLink
Log in

Developmental Biology of the Sea Urchin and Other Marine Invertebrates

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. David J. Carroll

    1. Department of Biochemistry and Molecular Genetics, Midwestern University, Glendale, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Stephen A. Stricker

    1. Department of Biology, University of New Mexico, Albuquerque, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2219)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (20 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Laboratory Culture and Mutagenesis of Amphioxus (Branchiostoma floridae)

    • Linda Z. Holland, Guang Li
    Pages 1-29

  3. Handling and Manipulation of Gametes and Embryos of the Annelidan Worm Pseudopotamilla occelata

    • Ryusaku Deguchi, Takeshi Nakano
    Pages 31-48

  4. The Starfish Asterina pectinifera: Collection and Maintenance of Adults and Rearing and Metamorphosis of Larvae

    • Naoyuki Murabe, Ei-ichi Okumura, Kazuyoshi Chiba, Enako Hosoda, Susumu Ikegami, Takeo Kishimoto
    Pages 49-68

  5. Experimental Tools to Study Regeneration in the Sea Anemone Nematostella vectensis

    • Aldine R. Amiel, Eric Röttinger
    Pages 69-80

  6. Staining and Tracking Methods for Studying Sponge Cell Dynamics

    • Carole Borchiellini, Sandie M. Degnan, Emilie Le Goff, Caroline Rocher, Amélie Vernale, Stephen Baghdiguian et al.
    Pages 81-97

  7. Microscopy Studies of Placozoans

    • Carolyn L. Smith, Tatiana D. Mayorova, Christine A. Winters, Thomas S. Reese, Sally P. Leys, Andreas Heyland
    Pages 99-118

  8. Identification of SH2 Domain-Mediated Protein Interactions that Operate at Fertilization in the Sea Star Patiria miniata

    • Lauren Bates, Emily Wiseman, Jamie Kitson, David J. Carroll
    Pages 119-135

  9. Marine Nemertean Worms for Immunoblotting Studies of Oocyte Aging

    • Stephen A. Stricker
    Pages 137-150

  10. Recovery of Sea Star Egg Cell Surface Proteins Released at Fertilization

    • Emily Wiseman, Lauren Bates, David J. Carroll
    Pages 151-161

  11. Quantifying Cell Proliferation During Regeneration of Aquatic Worms

    • Eduardo E. Zattara, B. Duygu Özpolat
    Pages 163-180

  12. In Situ Hybridization Techniques in the Homoscleromorph Sponge Oscarella lobularis

    • Laura Fierro-Constaín, Caroline Rocher, Florent Marschal, Quentin Schenkelaars, Nina Séjourné, Carole Borchiellini et al.
    Pages 181-194

  13. Methodology for Whole Mount and Fluorescent RNA In Situ Hybridization in Echinoderms: Single, Double, and Beyond

    • Margherita Perillo, Periklis Paganos, Maxwell Spurrell, Maria I. Arnone, Gary M. Wessel
    Pages 195-216

  14. Gene Editing in the Ascidian Phallusia mammillata and Tail Nerve Cord Formation

    • Alex McDougall, Celine Hebras, Isa Gomes, Remi Dumollard
    Pages 217-230

  15. Transcriptomic Analysis in the Sea Anemone Nematostella vectensis

    • Jacob F. Warner, Eric Röttinger
    Pages 231-240

  16. RNA Interference on Regenerating Holothurian Gut Tissues

    • Miosotis Alicea-Delgado, Samir A. Bello-Melo, José E. García-Arrarás
    Pages 241-252

  17. ATAC-Seq for Assaying Chromatin Accessibility Protocol Using Echinoderm Embryos

    • Marta S. Magri, Danila Voronov, Jovana RanÄ‘elović, Claudia Cuomo, Jose Luis Gómez-Skarmeta, Maria I. Arnone
    Pages 253-265

  18. Usage of the Sea Urchin Hemicentrotus pulcherrimus Database, HpBase

    • Sonoko Kinjo, Masato Kiyomoto, Takashi Yamamoto, Kazuho Ikeo, Shunsuke Yaguchi
    Pages 267-275

  19. Functional Studies of Trichoplax adhaerens Voltage-Gated Calcium Channel Activity

    • Julia Gauberg, Adriano Senatore, Andreas Heyland
    Pages 277-288

  20. A Bioinformatics Tutorial for Comparative Development Genomics in Diverse Meiofauna

    • Joseph L. Sevigny, Jon L. Norenburg, Francesca Leasi
    Pages 289-305
Back to top

Keywords

About this book

This detailed second edition presents a wide variety of marine invertebrate model systems, from cephalochordata to holothurians, along with novel experimental protocols for taking advantage of their unique properties. The techniques range from culturing the organisms to modifying their DNA. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Developmental Biology of the Sea Urchin and Other Marine Invertebrates: Methods and Protocols, Second Edition is an ideal guide for researchers working with these versatile organisms and for furthering our understanding of fundamental biological questions.

Editors and Affiliations

  • Department of Biochemistry and Molecular Genetics, Midwestern University, Glendale, USA

    David J. Carroll

  • Department of Biology, University of New Mexico, Albuquerque, USA

    Stephen A. Stricker

Bibliographic Information

  • Book Title: Developmental Biology of the Sea Urchin and Other Marine Invertebrates

  • Book Subtitle: Methods and Protocols

  • Editors: David J. Carroll, Stephen A. Stricker

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0974-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0973-6Published: 20 October 2020

  • Softcover ISBN: 978-1-0716-0976-7Published: 21 October 2021

  • eBook ISBN: 978-1-0716-0974-3Published: 19 October 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIII, 309

  • Number of Illustrations: 27 b/w illustrations, 48 illustrations in colour

  • Topics: Biological Techniques, Zoology, Developmental Biology

Publish with us

Policies and ethics

Back to top

Search

Navigation