Coronaviruses

1. Front Matter

   Pages i-xi

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2. Coronaviruses: An Updated Overview of Their Replication and Pathogenesis

   • Yuhang Wang, Matthew Grunewald, Stanley Perlman

   Pages 1-29

3. Coronavirus Detection, Discovery, and Evolution

   1. Front Matter

      Pages 31-31

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   2. Discovery of Novel Coronaviruses in Rodents

      • Theocharis Tsoleridis, Jonathan K. Ball

      Pages 33-40

   3. Detection and Discovery of Coronaviruses in Wild Bird Populations

      • Chantal J. Snoeck, Siamak Zohari

      Pages 41-53

   4. Competitive ELISA for the Detection of Serum Antibodies Specific for Middle East Respiratory Syndrome Coronavirus (MERS-CoV)

      • Shuetsu Fukushi

      Pages 55-65

   5. Whole-Genome Sequencing Protocols for IBV and Other Coronaviruses Using High-Throughput Sequencing

      • Graham L. Freimanis, Michael S. Oade

      Pages 67-74

4. Propagation and Titration of Coronaviruses

   1. Front Matter

      Pages 75-75

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   2. Isolation and Tissue Culture Adaptation of Porcine Deltacoronavirus: A Case Study

      • Hui Hu, Kwonil Jung, Scott P. Kenney, Linda J. Saif

      Pages 77-88

   3. The Preparation of Chicken Kidney Cell Cultures for Virus Propagation

      • Elena Lokhman, Srijana Rai, William Matthews

      Pages 89-95

   4. The Preparation of Chicken Tracheal Organ Cultures and Their Application for Ciliostasis Test, Growth Kinetics Studies, and Virus Propagation

      • Giulia Dowgier, Erica Bickerton

      Pages 97-106

   5. Isolation and Propagation of Coronaviruses in Embryonated Eggs

      • James S. Guy

      Pages 107-117

   6. Well-Differentiated Primary Mammalian Airway Epithelial Cell Cultures

      • Mitra Gultom, Laura Laloli, Ronald Dijkman

      Pages 119-134

   7. Quantification of Coronaviruses by Titration In Vitro and Ex Vivo

      • Giulia Dowgier, Helena J. Maier

      Pages 135-143

5. Manipulating the Genomes of Coronaviruses

   1. Front Matter

      Pages 145-145

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   2. Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitis Coronaviruses

      • Sarah Keep, Paul Britton, Erica Bickerton

      Pages 147-165

   3. In-Yeast Assembly of Coronavirus Infectious cDNA Clones Using a Synthetic Genomics Pipeline

      • Tran Thi Nhu Thao, Fabien Labroussaa, Nadine Ebert, Joerg Jores, Volker Thiel

      Pages 167-184

6. Studying Virus-Host Interactions

   1. Front Matter

      Pages 185-185

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   2. Proximity Labeling for the Identification of Coronavirus–Host Protein Interactions

      • Philip V’kovski, Silvio Steiner, Volker Thiel

      Pages 187-204

   3. Using Yeast to Identify Coronavirus–Host Protein Interactions

      • Stuart Weston, Matthew Frieman

      Pages 205-221

This detailed new edition provides a comprehensive collection of protocols applicable to all members of the Coronavirinae sub-family currently and that are also transferrable to other fields of virology. Beginning with a section on detection, discovery, and evolution, the volume continues with coverage of propagation and titration of coronaviruses, genome manipulation, study of virus-host interactions, as well as imaging coronavirus infections. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Coronaviruses: Methods and Protocols, Second Edition serves as a valuable guide to researchers working to identify and control viruses with increased potential to cross the species barrier and to develop the diagnostics, vaccines, and antiviral therapeutics that are required to manage future outbreaks in both humans and animals.

• Coronavirinae sub-family
• SARS
• Virus propagation
• Antiviral therapeutics
• Virus-host interactions
• Diagnostics
• Vaccine development

• The Pirbright Institute, Surrey, UK

  Helena J. Maier, Erica Bickerton

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