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Methods in Molecular Biology

DNA Modifications

Methods and Protocols

Editors: Ruzov, Alexey, Gering, Martin (Eds.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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  • ISBN 978-1-0716-0876-0
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About this book

This book provides an overview of methods and experimental protocols that are currently used to analyze the presence and abundance of non-canonical DNA nucleotides in different biological systems. Focusing particularly on the newly discovered and less studied DNA modifications that are enzymatically produced and are likely to play specific roles in various biological processes, the volume explores chromatography- and mass spectrometry-based techniques for the detection and quantification of DNA modifications, antibody-based approaches to study their spatial distribution in different cells and tissues, and methods to analyze their genomic distribution with the help of bioinformatics tools that interrogate the corresponding datasets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and comprehensive, DNA Modifications: Methods and Protocols serves as an ideal guide to research scientists and PhD students in this rapidly developing discipline, and, thus, will ultimately contribute to deciphering the roles of non-canonical DNA nucleotides in different biological systems.

Table of contents (34 chapters)

Table of contents (34 chapters)
  • Modified Forms of Cytosine in Eukaryotes: DNA (De)methylation and Beyond

    Pages 3-13

    Eleftheriou, Maria (et al.)

  • Evidence for Noncytosine Epigenetic DNA Modifications in Multicellular Eukaryotes: An Overview

    Pages 15-25

    Lowe, Paige (et al.)

  • Mass Spectrometry-Based Analysis of DNA Modifications: Potential Applications in Basic Research and Clinic

    Pages 27-35

    Olinski, Ryszard (et al.)

  • Mapping DNA Methylation in Mammals: The State of the Art

    Pages 37-50

    Lentini, Antonio (et al.)

  • Detection of Modified Bases in Bacteriophage Genomic DNA

    Pages 53-66

    Lee, Yan-Jiun (et al.)

Buy this book

eBook  
  • ISBN 978-1-0716-0876-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
Hardcover £199.99
price for United Kingdom (gross)
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Bibliographic Information

Bibliographic Information
Book Title
DNA Modifications
Book Subtitle
Methods and Protocols
Editors
  • Alexey Ruzov
  • Martin Gering
Series Title
Methods in Molecular Biology
Series Volume
2198
Copyright
2021
Publisher
Springer US
Copyright Holder
The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-0716-0876-0
DOI
10.1007/978-1-0716-0876-0
Hardcover ISBN
978-1-0716-0875-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVI, 495
Number of Illustrations
27 b/w illustrations, 70 illustrations in colour
Topics