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Plant Endosomes

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2177)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xi
  2. Analysis of Membrane Proteins Transport from Endosomal Compartments to Vacuoles

    • Mengqian Luo, Ying Zhu, Zhiqi Liu, Liwen Jiang
    Pages 15-21
  3. Degradation of Abscisic Acid Receptors Through the Endosomal Pathway

    • Borja Belda-Palazón, Pedro L. Rodriguez
    Pages 35-48
  4. Imaging Plant Cells by High-Pressure Freezing and Serial block-face scanning electron microscopy

    • Kirk Czymmek, Abhilash Sawant, Kaija Goodman, Janice Pennington, Pal Pedersen, Mrinalini Hoon et al.
    Pages 69-81
  5. Subcellular Localization of PI3P in Arabidopsis

    • Han Nim Lee, Hyera Jung, Taijoon Chung
    Pages 109-118
  6. Immunopurification of Intact Endosomal Compartments for Lipid Analyses in Arabidopsis

    • Yoko Ito, Magali Grison, Nicolas Esnay, Laetitia Fouillen, Yohann Boutté
    Pages 119-141
  7. Isolation and Glycomic Analysis of Trans-Golgi Network Vesicles in Plants

    • Guangxi Ren, Michel Ruiz Rosquete, Angelo G. Peralta, Sivakumar Pattathil, Michael G. Hahn, Thomas Wilkop et al.
    Pages 153-167
  8. Back Matter

    Pages 199-200

About this book

This second edition details techniques for the study of cargo trafficking through endosomes. New and updated chapters guide readers through methods and protocols on the structural aspects of plant endosomes, combined biochemical, omics, imaging approaches to study the dynamics and contents of endosomal compartments. Additional chapters are dedicated to the analysis of lipids on endosomes and the identification and analysis of lipid binding proteins and lipid-binding domains relevant for the study of plant endosomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Plant Endosomes: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.


Editors and Affiliations

  • Department of Botany and Laboratory of Cell and Molecular Biology, University of Wisconsin-Madison, Madison, USA

    Marisa S. Otegui

Bibliographic Information

  • Book Title: Plant Endosomes

  • Book Subtitle: Methods and Protocols

  • Editors: Marisa S. Otegui

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0767-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0766-4Published: 07 July 2020

  • Softcover ISBN: 978-1-0716-0769-5Published: 07 July 2021

  • eBook ISBN: 978-1-0716-0767-1Published: 06 July 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 200

  • Number of Illustrations: 10 b/w illustrations, 31 illustrations in colour

  • Topics: Plant Sciences, Cell Biology

Buy it now

Buying options

eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access