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Photoswitching Proteins

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Dominik Niopek

    1. IPMB and BioQuant, Heidelberg University, Heidelberg, Germany

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2173)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Studying Neuronal Function Ex Vivo Using Optogenetic Stimulation and Patch Clamp

    • Ayla Aksoy-Aksel, Julien Genty, Martin Zeller, Ingrid Ehrlich
    Pages 1-20

  3. Optogenetic Techniques for Manipulating and Sensing G Protein-Coupled Receptor Signaling

    • Nohely Abreu, Joshua Levitz
    Pages 21-51

  4. Melanopsin for Time-Controlling Activation of Astrocyte –Neuron Networks

    • Sara Mederos, Candela González-Arias, Gertrudis Perea
    Pages 53-69

  5. Near-infrared Deep Brain Stimulation in Living Mice

    • Shuo Chen
    Pages 71-82

  6. Lab-Scale Production of Recombinant Adeno-Associated Viruses (AAV) for Expression of Optogenetic Elements

    • Janina Haar, Chiara Krämer, Dirk Grimm
    Pages 83-100

  7. AAV-Mediated Gene Delivery to Foveal Cones

    • Stéphane Bertin, Elena Brazhnikova, Céline Jaillard, José-Alain Sahel, Deniz Dalkara
    Pages 101-112

  8. Engineering Optogenetic Protein Analogs

    • Bei Liu, Daniel J. Marston, Klaus M. Hahn
    Pages 113-126

  9. Optogenetic Control of Nucleocytoplasmic Protein Transport

    • Daniel Weis, Barbara Di Ventura
    Pages 127-136

  10. Light-Inducible CRISPR Labeling

    • Mareike D. Hoffmann, Felix Bubeck, Dominik Niopek
    Pages 137-150

  11. Optogenetic Control of Gene Expression Using Cryptochrome 2 and a Light-Activated Degron

    • Carmen N. Hernández-Candia, Chandra L. Tucker
    Pages 151-158

  12. Optogenetic Downregulation of Protein Levels to Control Programmed Cell Death in Mammalian Cells with a Dual Blue-Light Switch

    • Patrick Fischbach, Patrick Gonschorek, Julia Baaske, Jamie A. Davies, Wilfried Weber, Matias D. Zurbriggen
    Pages 159-170

  13. Tracing Reversible Light-Induced Chromatin Binding with Near-infrared Fluorescent Proteins

    • Anne Rademacher, Fabian Erdel, Jorge Trojanowski, Karsten Rippe
    Pages 171-188

  14. Construction of a Multiwell Light-Induction Platform for Traceless Control of Gene Expression in Mammalian Cells

    • Maysam Mansouri, Samson Lichtenstein, Tobias Strittmatter, Peter Buchmann, Martin Fussenegger
    Pages 189-199

  15. Dual Activation of cAMP Production Through Photostimulation or Chemical Stimulation

    • Nyla Naim, Jeff M. Reece, Xuefeng Zhang, Daniel L. Altschuler
    Pages 201-216

  16. Synthesis of a Light-Controlled Phytochrome-Based Extracellular Matrix with Reversibly Adjustable Mechanical Properties

    • Maximilian Hörner, Philipp Hoess, Ramona Emig, Balder Rebmann, Wilfried Weber
    Pages 217-231

  17. Design and Application of Light-Regulated Receptor Tyrosine Kinases

    • Stephanie Kainrath, Harald Janovjak
    Pages 233-246

  18. All-Optical Miniaturized Co-culture Assay of Voltage-Gated Ca2+ Channels

    • Viviana Agus, Harald Janovjak
    Pages 247-260

  19. Optogenetics and CRISPR: A New Relationship Built to Last

    • Jan Mathony, Mareike D. Hoffmann, Dominik Niopek
    Pages 261-281

  20. Back Matter

    Pages 283-287
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Keywords

About this book

This volume provides cutting edge protocols for using optogenetic and opto-chemical techniques across a wide spectrum of applications. Chapters guide readers through methods in neuro-optogenetics, including methods to dissect local and long-range connectivity ex vivo, investigate G-protein coupled receptors, study astrocyte-neuron networks in vitro and in vivo, employ upconversion nanoparticles for transcranial optogenetic stimulation, and use Adeno-associated virus (AAV) for delivery of optogenetic constructs. At the same time, this book covers a range of methods for non-neuronal optogenetics, including various applications of light-oxygen-voltage domains or cryptochromes for control of nucleocytoplasmic transport, transgene expression and chromatin recruitment. The book further presents protocols for traceless light-control of gene expression with melanopsin, light-inducible cAMP production, use of light-regulated receptor tyrosine kinases (Opto-RTKs), synthesis of light-dependent biomaterials, and highlights emerging tools at the intersection of optogenetics and CRISPR. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Photoswitching Proteins: Methods and Protocols aims to provide a comprehensive list of methods and tools to investigate highly dynamic pathways and behaviors in cells and animals using optogenetics.

Editors and Affiliations

  • IPMB and BioQuant, Heidelberg University, Heidelberg, Germany

    Dominik Niopek

Bibliographic Information

  • Book Title: Photoswitching Proteins

  • Book Subtitle: Methods and Protocols

  • Editors: Dominik Niopek

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0755-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0754-1Published: 11 July 2020

  • Softcover ISBN: 978-1-0716-0757-2Published: 11 July 2021

  • eBook ISBN: 978-1-0716-0755-8Published: 10 July 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 287

  • Number of Illustrations: 2 b/w illustrations, 66 illustrations in colour

  • Topics: Neurosciences, Physiology, Protein Science, Biological Techniques

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