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Basophils and Mast Cells

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Bernhard F. Gibbs

    1. Division of Experimental Allergology and Immunodermatology, Department of Human Medicine, School of Medicine and Health Sciences, Carl von Ossietzky University of Oldenburg, Oldenburg, Germany

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  2. Franco H. Falcone

    1. Institute for Parasitology, Justus Liebig University Giessen, Biomedizinisches Forschungszentrum Seltersberg (BFS), Giessen, Germany

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2163)

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Table of contents (30 protocols)

  1. Front Matter

    Pages i-xviii
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  2. Introduction and Historical Background

    1. Front Matter

      Pages 1-1
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    2. Paradigm Shifts in Mast Cell and Basophil Biology and Function: An Emerging View of Immune Regulation in Health and Disease

      • Ana Olivera, Juan Rivera
      Pages 3-31

  3. Obtaining the Cells: Purification, Culture, and Counting

    1. Front Matter

      Pages 33-33
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    2. Purification of Basophils from Peripheral Human Blood

      • Franco H. Falcone, Bernhard F. Gibbs
      Pages 35-48

    3. Mast Cell Purification Protocols

      • Jasmine Farrington, Elizabeth P. Seward, Peter T. Peachell
      Pages 49-56

    4. Generation of a Human Allergic Mast Cell Phenotype from CD133+ Stem Cells

      • Hans Jürgen Hoffmann
      Pages 57-62

    5. Generation and Culture of Peripheral CD34+ Stem Cell-Derived Mast Cells (PSCMCs)

      • Yanyan Luo, Stefan Frischbutter, Jörg Scheffel, Frank Siebenhaar
      Pages 63-67

    6. Modulating the Human Basophil Phenotype During Its Development and Maturation: Basophils Derived from In Vitro Cultures of CD34+ Progenitor Cells

      • Donald MacGlashan Jr.
      Pages 69-83

    7. Generation of Mast Cells from Murine Stem Cell Progenitors

      • Emily J. Swindle
      Pages 85-89

    8. Integration of the Human Dermal Mast Cell into the Organotypic Co-culture Skin Model

      • Jonghui Kim, Sven Guhl, Magda Babina, Torsten Zuberbier, Metin Artuc
      Pages 91-107

    9. The Absolute Basophil Count

      • Elena Borzova
      Pages 109-124

  4. Basophil/Mast Cell Lines

    1. Front Matter

      Pages 125-125
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    2. Mast Cell and Basophil Cell Lines: A Compendium

      • Egle Passante
      Pages 127-144

    3. Use of Humanized RBL Reporter Systems for the Detection of Allergen-Specific IgE Sensitization in Human Serum

      • Daniel Wan, Xiaowei Wang, Ryosuke Nakamura, Marcos J. C. Alcocer, Franco H. Falcone
      Pages 145-153

    4. Use of Humanized Fluorescent Reporter Cell Line RBL NFAT-DsRed for the Detection of Allergen-Specific IgE in Patient Sera Using Allergen Microarrays

      • Marina Kalli, Marcos J. C. Alcocer, Andrew J. Blok, Franco H. Falcone
      Pages 155-162

    5. NPY-mRFP Rat Basophilic Leukemia (RBL) Reporter: A Novel, Fast Reporter of Basophil/Mast Cell Degranulation

      • Nafal J. S. Barwary, Daniel Wan, Franco H. Falcone
      Pages 163-170

    6. Use of Engineered Nanoparticles (ENPs) for the Study of High-Affinity IgE FcεRI Receptor Engagement and Rat Basophilic Leukemia (RBL) Cell Degranulation

      • Walla Alelwani, Raed A. Alharbi, Daniel Wan, Driton Vllasaliu, Franco. H. Falcone, Snow Stolnik
      Pages 171-180

  5. Diagnostic Applications

    1. Front Matter

      Pages 181-181
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    2. Flow Cytometric Allergy Diagnosis: Basophil Activation Techniques

      • Chris H. Bridts, Vito Sabato, Christel Mertens, Margo M. Hagendorens, Luc S. De Clerck, Didier G. Ebo
      Pages 183-195
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Keywords

About this book

This second edition provides updated and new chapters to build on and extend the strengths of the first edition. Chapters guide readers through basic biology of basophils, obtaining the cells by purification, culture of stem cells progenitors, peripheral CD34+ stem cell-derived mast cells, basophils from CD34+ progenitors, diagnostic applications, gene expression patterns in basophils, roles of basophils in different asthma phenotypes, knockout, and disease models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Basophils and Mast Cells: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Division of Experimental Allergology and Immunodermatology, Department of Human Medicine, School of Medicine and Health Sciences, Carl von Ossietzky University of Oldenburg, Oldenburg, Germany

    Bernhard F. Gibbs

  • Institute for Parasitology, Justus Liebig University Giessen, Biomedizinisches Forschungszentrum Seltersberg (BFS), Giessen, Germany

    Franco H. Falcone

Bibliographic Information

  • Book Title: Basophils and Mast Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Bernhard F. Gibbs, Franco H. Falcone

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0696-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0695-7Published: 07 August 2020

  • Softcover ISBN: 978-1-0716-0698-8Published: 08 August 2021

  • eBook ISBN: 978-1-0716-0696-4Published: 06 August 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XVIII, 380

  • Number of Illustrations: 2 b/w illustrations, 48 illustrations in colour

  • Topics: Immunology, Cell Biology

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