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Nanoparticles in Biology and Medicine

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Enrico Ferrari

    1. College of Science, School of Life Sciences, University of Lincoln, Brayford Pool, Lincoln, UK

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  2. Mikhail Soloviev

    1. Centre for Biomedical Sciences, Department of Biological Sciences, Royal Holloway University of London, Egham, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2118)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xvi
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  2. Synthesis of Nanoparticles and Their Applications in Biology and Medicine

    1. Front Matter

      Pages 1-1
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    2. Procedures for the Synthesis and Capping of Metal Nanoparticles

      • Claudia Gutiérrez-Wing, J. Jesús Velázquez-Salazar, Miguel José-Yacamán
      Pages 3-20

    3. Antimicrobial Applications of Silver Nanoparticles to E. coli Colony Biofilms

      • James P. McEvoy, Kayra Genc, Priya Loi, William J. Walker
      Pages 21-28

    4. Elastins-Based Antimicrobial Particles for Delivery of Bioactive Compounds

      • Raul Machado, André da Costa, Ana Margarida Pereira, José Carlos Rodriguez-Cabello, Margarida Casal
      Pages 29-43

    5. Biomimetic Lipid Polymer Nanoparticles for Drug Delivery

      • Ana Maria Carmona-Ribeiro
      Pages 45-60

    6. Crotamine Cell-Penetrating Nanocarriers: Cancer-Targeting and Potential Biotechnological and/or Medical Applications

      • Mirian A. F. Hayashi, Joana Darc Campeiro, Lucas Carvalho Porta, Brian Szychowski, Wendel Andrade Alves, Eduardo B. Oliveira et al.
      Pages 61-89

    7. Preparation of Lipid–Peptide–DNA (LPD) Nanoparticles and Their Use for Gene Transfection

      • Fan Zhang, Hao-Ying Li
      Pages 91-98

    8. Preparation of Hyaluronic Acid-Based Nanoparticles for Macrophage-Targeted MicroRNA Delivery and Transfection

      • Neha N. Parayath, Mansoor M. Amiji
      Pages 99-110

    9. Formulation and Characterization of Antithrombin Perfluorocarbon Nanoparticles

      • Alexander J. Wilson, Qingyu Zhou, Ian Vargas, Rohun Palekar, Ryan Grabau, Hua Pan et al.
      Pages 111-120

    10. Biomedical In Vivo Studies with ORMOSIL Nanoparticles Containing Active Agents

      • Sona Gandhi, Indrajit Roy
      Pages 121-138

    11. Preparation of Spray-Dried Nanoparticles for Efficient Drug Delivery to the Lungs

      • Hao-Ying Li, Fan Zhang
      Pages 139-145

    12. Synthesis and Evaluation of Airway-Targeted PLGA-PEG Nanoparticles for Drug Delivery in Obstructive Lung Diseases

      • Neeraj Vij
      Pages 147-154

    13. Synthesis and Evaluation of Dendrimers for Autophagy Augmentation and Alleviation of Obstructive Lung Diseases

      • Neeraj Vij
      Pages 155-164

    14. Synthesis of Poly(2-Hydroxyethyl Methacrylate) (PHEMA)-Based Superparamagnetic Nanoparticles for Biomedical and Pharmaceutical Applications

      • Rajesh K. Saini, Jaya Bajpai, Anil K. Bajpai
      Pages 165-174

  3. Focus on the Nanoparticles Derivatization, Bio-Interface, and Nanotoxicity

    1. Front Matter

      Pages 175-175
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    2. The Molecular Dynamics Simulation of Peptides on Gold Nanosurfaces

      • Danilo Roccatano
      Pages 177-197

    3. Protein Immobilization on Gold Nanoparticles: Quantitative Analysis

      • Evan Decker, Chunsheng Bai, Lauren Nelless, Enrico Ferrari, Mikhail Soloviev
      Pages 199-211

    4. Oriented Immobilization on Gold Nanoparticles of a Recombinant Therapeutic Zymogen

      • Elina Dosadina, Celetia Agyeiwaa, William Ferreira, Simon Cutting, Abdullah Jibawi, Enrico Ferrari et al.
      Pages 213-225

    5. Directed and Modular Protein Immobilization on Gold and Silver Nanoparticles

      • Angela Saccardo, Wenwei Ma, Mikhail Soloviev, Enrico Ferrari
      Pages 227-234
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Keywords

About this book

This fully updated volume presents a wide range of methods for synthesis, surface modification, characterization and application of nano-sized materials (nanoparticles) in the life science and medical fields, with a focus on drug delivery and diagnostics. Beginning with a section on the synthesis of nanoparticles and their applications, the book continues with detailed chapters on nanoparticle derivatization, bio-interface, and nanotoxicity, as well as nanoparticle characterization and advanced methods development. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Nanoparticles in Biology and Medicine: Methods and Protocols, Second Edition serves as an ideal guide for scientistsat all levels of expertise to a wide range of biomedical and pharmaceutical applications including functional protein studies, drug delivery, immunochemistry, imaging, and more.

Editors and Affiliations

  • College of Science, School of Life Sciences, University of Lincoln, Brayford Pool, Lincoln, UK

    Enrico Ferrari

  • Centre for Biomedical Sciences, Department of Biological Sciences, Royal Holloway University of London, Egham, UK

    Mikhail Soloviev

Bibliographic Information

  • Book Title: Nanoparticles in Biology and Medicine

  • Book Subtitle: Methods and Protocols

  • Editors: Enrico Ferrari, Mikhail Soloviev

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0319-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0318-5Published: 10 March 2020

  • Softcover ISBN: 978-1-0716-0321-5Published: 10 March 2021

  • eBook ISBN: 978-1-0716-0319-2Published: 09 March 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XVI, 446

  • Number of Illustrations: 62 b/w illustrations, 103 illustrations in colour

  • Topics: Pharmaceutical Sciences/Technology, Nanotechnology

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