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RNA Interference and CRISPR Technologies

Technical Advances and New Therapeutic Opportunities

  • Book
  • © 2020

Overview

Editors:
  1. Mouldy Sioud

    1. Department of Cancer Immunology, Institute for Cancer Research, Oslo University Hospital-Radiumhospitalet, Oslo, Norway

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key note and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2115)

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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. RNA and CRISPR Interferences: Past, Present, and Future Perspectives

    • Mouldy Sioud
    Pages 1-22

  3. Chemical Modifications in RNA Interference and CRISPR/Cas Genome Editing Reagents

    • Kim A. Lennox, Mark A. Behlke
    Pages 23-55

  4. Preparation, Determination of Activity, and Biodistribution of Cholesterol-Containing Nuclease-Resistant siRNAs In Vivo

    • Ivan V. Chernikov, Mariya I. Meschaninova, Elena L. Chernolovskaya
    Pages 57-77

  5. Multifunctional Nanodelivery Platform for Maximizing Nucleic Acids Combination Therapy

    • Seung Koo Lee, Benedict Law, Ching-Hsuan Tung
    Pages 79-90

  6. PAMAM Dendrimers as a Delivery System for Small Interfering RNA

    • Shiva Kheiriabad, Maryam Ghaffari, Jafar Ezzati Nazhad Dolatabadi, Michael R. Hamblin
    Pages 91-106

  7. Delivery of Functional Small RNAs via Extracellular Vesicles In Vitro and In Vivo

    • Duo Zhang, Heedoo Lee, Yang Jin
    Pages 107-117

  8. Optimized siRNA Delivery into Primary Immune Cells Using Electroporation

    • Mouldy Sioud
    Pages 119-131

  9. Synthesis and Evaluation of Caged siRNAs with Single cRGD Modification for Photoregulating RNA Interference

    • Lijia Yu, Duanwei Liang, Nannan Jing, Changmai Chen, Xinjing Tang
    Pages 133-161

  10. Exploring 5′-Biotinylation of the Sense Strand to Improve siRNA Specificity and Potency

    • Anne Mobergslien, Mouldy Sioud
    Pages 163-170

  11. In Vivo Delivery of Cassettes Encoding Anti-HBV Primary MicroRNAs Using an Ancestral Adeno-Associated Viral Vector

    • Njabulo Mnyandu, Patrick Arbuthnot, Mohube Betty Maepa
    Pages 171-183

  12. Generating DNA Expression Cassettes Encoding Multimeric Artificial MicroRNA Precursors

    • Fiona T. van den Berg, Abdullah Ely, Patrick Arbuthnot
    Pages 185-197

  13. MiR-302-Mediated Somatic Cell Reprogramming and Method for Generating Tumor-Free iPS Cells Using miR-302

    • Shi-Lung Lin, Jack S. Chen, Shao-Yao Ying
    Pages 199-219

  14. Urinary MicroRNAs as Emerging Class of Noninvasive Biomarkers

    • Jaroslav Juracek, Ondrej Slaby
    Pages 221-247

  15. Improving Dendritic Cell Cancer Vaccine Potency Using RNA Interference

    • Stein Sæbøe-Larssen, Mouldy Sioud
    Pages 249-258

  16. Unleashing the Therapeutic Potential of Dendritic and T Cell Therapies Using RNA Interference

    • Mouldy Sioud
    Pages 259-280

  17. Harnessing the Antiviral-Type Responses Induced by Immunostimulatory siRNAs for Cancer Immunotherapy

    • Per Ole Iversen, Mouldy Sioud
    Pages 281-287

  18. Cancer Immunotherapy: Targeting Tumor-Associated Macrophages by Gene Silencing

    • Karin Zins, Dietmar Abraham
    Pages 289-325

  19. Use of RNA Interference with TCR Transfer to Enhance Safety and Efficiency

    • Nicholas Paul Casey, Jon Amund Kyte, Hiroshi Fujiwara
    Pages 327-349

  20. CRISPR/Cas9 Guide RNA Design Rules for Predicting Activity

    • Kasidet Hiranniramol, Yuhao Chen, Xiaowei Wang
    Pages 351-364
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Keywords

About this book

This volume explores the uses of RNAi and CRISPR interferences as a general method for inhibiting gene expression, with focus on their biological functions, design, chemical modifications, delivery, and preclinical/clinical applications. In addition to relevant backgrounds, the chapters in this book discuss simple and accurate protocols dealing with the latest advances in RNAi and CRISPR applications and look at how these methods differ from one another. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Comprehensive and cutting-edge, RNA Interference and CRISPR Technologies: Technical Advances and New Therapeutic Opportunities is a valuable resource for any scientist, teachers, graduate student, postdoc,and clinician interested in this field. This book also benefits anyone in research and development in biotech and pharmaceutical companies who want to understand more about these technologies, and their applications in biology and medicine. 

Editors and Affiliations

  • Department of Cancer Immunology, Institute for Cancer Research, Oslo University Hospital-Radiumhospitalet, Oslo, Norway

    Mouldy Sioud

Bibliographic Information

  • Book Title: RNA Interference and CRISPR Technologies

  • Book Subtitle: Technical Advances and New Therapeutic Opportunities

  • Editors: Mouldy Sioud

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0290-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0289-8Published: 01 February 2020

  • Softcover ISBN: 978-1-0716-0292-8Published: 01 February 2021

  • eBook ISBN: 978-1-0716-0290-4Published: 31 January 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 486

  • Number of Illustrations: 22 b/w illustrations, 48 illustrations in colour

  • Topics: Gene Expression, Genetics and Genomics

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