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T-Cell Receptor Signaling

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Chaohong Liu

    1. Department of Pathogen Biology, Huazhong University of Science and Technology, Wuhan, China

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2111)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Exploration of T-Cell Diversity Using Mass Cytometry

    • Kaitlin C. O’Boyle, Takuya Ohtani, Sasikanth Manne, Bertram Bengsch, Sarah E. Henrickson, E. John Wherry et al.
    Pages 1-20

  3. A Carrier Strategy for Mass Cytometry Analysis of Small Numbers of Cells

    • Xian Jia, Xiaojuan Zhou, Haiping Zheng, Shan Jiang, Jiannan Weng, Lei Huang et al.
    Pages 21-33

  4. Simultaneous Measurement of Surface Proteins and Gene Expression from Single Cells

    • Jiadi Luo, Carla A. Erb, Kong Chen
    Pages 35-46

  5. Analysis of Transcriptional Profiling of Immune Cells at the Single-Cell Level

    • Annabel Ferguson, Kong Chen
    Pages 47-57

  6. CRISPR/Cas9-Based Genetic Screening to Study T-Cell Function

    • Wanjing Shang, Fei Wang, Qi Zhu, Liangyu Wang, Haopeng Wang
    Pages 59-70

  7. Preferential Expansion of CD4+Foxp3+ Regulatory T Cells (Tregs) In Vitro by Tumor Necrosis Factor

    • Chon-Kit Chou, Xin Chen
    Pages 71-78

  8. In Vitro Differentiation of CD4+ T Cell Effector and Regulatory Subsets

    • Jaclyn R. Espinosa, Joshua D. Wheaton, Maria Ciofani
    Pages 79-89

  9. CD4+ T-Cell Differentiation In Vitro

    • Wenyong Yang, Xueying Chen, Hongbo Hu
    Pages 91-99

  10. Characterization of Immune Cell Subset Expansion in Response to Therapeutic Treatment in Mice

    • Jakub Tomala, Jamie B. Spangler
    Pages 101-114

  11. Primary T-Cell Transduction to Study Follicular Helper T-Cell Differentiation

    • Yang Zhang, Xuehui Long, Xiaoming Wang
    Pages 115-126

  12. In Vitro Generation of Stem Cell Memory-Like T Cells from Activated T Cells

    • Makoto Ando, Mari Ikeda, Akihiko Yoshimura, Taisuke Kondo
    Pages 127-139

  13. Artificial Antigen Presentosomes for T Cell Activation

    • Yi-Geng Pang, Chien-Chung Chang
    Pages 141-151

  14. Imaging Chimeric Antigen Receptor (CAR) Activation

    • Kendra A. Libby, Xiaolei Su
    Pages 153-160

  15. Assessing the Impact of Phytochemicals on Immune Checkpoints: Implications for Cancer Immunotherapy

    • Melanie R. Power Coombs, David W. Hoskin
    Pages 161-173

  16. Assessment of Immune Protective T Cell Repertoire in Humans Immunized with Novel Tuberculosis Vaccines

    • Mangalakumari Jeyanathan, Zhou Xing
    Pages 175-192

  17. Retroviral Gene Transduction into T Cell Progenitors for Analysis of T Cell Development in the Thymus

    • Ryunosuke Muro, Hiroshi Takayanagi, Takeshi Nitta
    Pages 193-203

  18. Testing the Efficiency and Kinetics of Negative Selection Using Thymic Slices

    • Tyng-An Zhou, Chia-Lin Hsu, Ivan Lilyanov Dzhagalov
    Pages 205-219

  19. Investigating T Cell Receptor Signals In Situ by Two-Photon Microscopy of Thymocytes Expressing Genetic Reporters in Low-Density Chimeras

    • Marilaine Fournier, Mengqi Dong, Heather J. Melichar
    Pages 221-238

  20. An Integrated Strategy for Identifying Targets of Ubiquitin-Mediated Degradation in CD4+ T Cells

    • Natania S. Field, Claire E. O’Leary, Joseph M. Dybas, Hua Ding, Paula M. Oliver
    Pages 239-256
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Keywords

About this book

This volume provides current and new advanced methods and protocols to study T cells. Chapters guide readers through T cell diversity using mass cytometry, analyzing T cells from single cell level, CRISPR/Cas9 techniques to study the T cell activation, techniques to study subsets of Tcell’s, procedures to study artificial antigen presentosomes for T cell activation, techniques to study the T cell development, two-photon microscopy, and MAIT cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, T-Cell Receptor Signaling: Methods and Protocols aims to provide a wide range of approaches and be an invaluable resource for present and future generations of T cell researchers.

Editors and Affiliations

  • Department of Pathogen Biology, Huazhong University of Science and Technology, Wuhan, China

    Chaohong Liu

Bibliographic Information

  • Book Title: T-Cell Receptor Signaling

  • Book Subtitle: Methods and Protocols

  • Editors: Chaohong Liu

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0266-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0265-2Published: 14 January 2020

  • Softcover ISBN: 978-1-0716-0268-3Published: 14 January 2021

  • eBook ISBN: 978-1-0716-0266-9Published: 13 January 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 296

  • Number of Illustrations: 16 b/w illustrations, 48 illustrations in colour

  • Topics: Biochemistry, general, Immunology

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