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MERS Coronavirus

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Rahul Vijay

    1. Department of Microbiology and Immunology, University of Iowa, Iowa City, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2099)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
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  2. Evolution and Entry of MERS-Coronavirus

    1. Front Matter

      Pages 1-1
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    2. Studying Evolutionary Adaptation of MERS-CoV

      • Michael Letko, Vincent Munster
      Pages 3-8

    3. Evaluating MERS-CoV Entry Pathways

      • Enya Qing, Michael P. Hantak, Gautami G. Galpalli, Tom Gallagher
      Pages 9-20

    4. Biochemical Characterization of Middle East Respiratory Syndrome Coronavirus Spike Protein Proteolytic Processing

      • Gary R. Whittaker, Jean K. Millet
      Pages 21-37

    5. Crystallization and Structural Determination of the Receptor-Binding Domain of MERS-CoV Spike Glycoprotein

      • Haixia Zhou, Shuyuan Zhang, Xinquan Wang
      Pages 39-50

  3. Genetic Alteration and Structural Determination of MERS-Coronavirus Proteins

    1. Front Matter

      Pages 51-51
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    2. Bacterial Artificial Chromosome-Based Lambda Red Recombination with the I-SceI Homing Endonuclease for Genetic Alteration of MERS-CoV

      • Anthony R. Fehr
      Pages 53-68

    3. Deducing the Crystal Structure of MERS-CoV Helicase

      • Sheng Cui, Wei Hao
      Pages 69-85

  4. Quantitation of Virus and Anti-Viral Factors

    1. Front Matter

      Pages 87-87
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    2. Antigen Capture Enzyme-Linked Immunosorbent Assay for Detecting Middle East Respiratory Syndrome Coronavirus in Humans

      • Joshua Fung, Susanna K. P. Lau, Patrick C. Y. Woo
      Pages 89-97

    3. Quantification of the Middle East Respiratory Syndrome-Coronavirus RNA in Tissues by Quantitative Real-Time RT-PCR

      • Abdullah Algaissi, Anurodh S. Agrawal, Anwar M. Hashem, Chien-Te K. Tseng
      Pages 99-106

    4. Evaluation of MERS-CoV Neutralizing Antibodies in Sera Using Live Virus Microneutralization Assay

      • Abdullah Algaissi, Anwar M. Hashem
      Pages 107-116

    5. Generation of MERS-CoV Pseudotyped Viral Particles for the Evaluation of Neutralizing Antibodies in Mammalian Sera

      • Abdulrahman Almasaud, Naif Khalaf Alharbi, Anwar M. Hashem
      Pages 117-126

    6. Qualitative and Quantitative Determination of MERS-CoV S1-Specific Antibodies Using ELISA

      • Sawsan S. Al-amri, Anwar M. Hashem
      Pages 127-133

  5. Mouse Models for MERS –Coronavirus

    1. Front Matter

      Pages 135-135
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    2. Genetically Engineering a Susceptible Mouse Model for MERS-CoV-Induced Acute Respiratory Distress Syndrome

      • Sarah R. Leist, Adam S. Cockrell
      Pages 137-159

    3. Development of a Mouse-Adapted MERS Coronavirus

      • Kun Li, Paul B. McCray Jr.
      Pages 161-171

    4. Metabolite, Protein, and Lipid Extraction (MPLEx): A Method that Simultaneously Inactivates Middle East Respiratory Syndrome Coronavirus and Allows Analysis of Multiple Host Cell Components Following Infection

      • Carrie D. Nicora, Amy C. Sims, Kent J. Bloodsworth, Young-Mo Kim, Ronald J. Moore, Jennifer E. Kyle et al.
      Pages 173-194

    5. Evaluation of Activation and Inflammatory Activity of Myeloid Cells During Pathogenic Human Coronavirus Infection

      • Rudragouda Channappanavar, Stanley Perlman
      Pages 195-204
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Keywords

About this book

This volume provides various techniques and methodologies currently used in the study of MERS-CoV. Chapters are divided into four parts detailing evolution and entry of MERS-coronavirus, genetic alteration and structural determination of MERS-coronavirus proteins, quantitation of virus and anti-viral factors, and mouse models for MERS -coronavirus.   Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, MERS Coronavirus: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Department of Microbiology and Immunology, University of Iowa, Iowa City, USA

    Rahul Vijay

Bibliographic Information

  • Book Title: MERS Coronavirus

  • Book Subtitle: Methods and Protocols

  • Editors: Rahul Vijay

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0211-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0210-2Published: 28 December 2019

  • Softcover ISBN: 978-1-0716-0213-3Published: 28 December 2020

  • eBook ISBN: 978-1-0716-0211-9Published: 27 December 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 224

  • Number of Illustrations: 13 b/w illustrations, 27 illustrations in colour

  • Topics: Immunology, Virology

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