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Methods in Molecular Biology
cover

Neutrophil

Methods and Protocols

Editors: Quinn, Mark T., DeLeo, Frank (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-07-160154-9
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  • ISBN 978-1-07-160153-2
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About this book

Neutrophils, the most abundant white cells in humans, serve as the primary cellular defense against infection. This volume provides a concise set of protocols for assessing basic neutrophil functions and investigating specialized areas in neutrophil biology. Each of the protocols is written by leading researchers in the field and includes hints for success, as well as guidance for troubleshooting. In the third edition of Neutrophils: Methods and Protocols, chapters retained from previous editions have been have been updated and include many new approaches.  In addition, the Third Edition contains a number of new chapters that were not included in the first or second editions.  Part I contains overviews of neutrophil biology, function, and disorders.  Part II describes commonly used methods to isolate neutrophils from humans and other animal species. Part III details methods for investigating chemotaxis, transmigration, phagocytosis, and bactericidal activity.  Part IV includes protocols that measure neutrophil apoptosis, calcium signal transduction, degranulation and detection of cytoplasmic granules, gene expression, transcription factors, and apoptosis.  Part V provides multiple assays for measuring production of intracellular and/or extracellular reactive oxygen species, and a method that details use of the cell-free NADPH oxidase assay, an iconic assay for studies of the neutrophil NADPH oxidase.  Part VI provides chapters that describe how to analyze formation and function of neutrophil extracellular traps. Neutrophil Methods and Protocols: Third Edition is a comprehensive source for detailed explanations and applications of the most modern methodological advances in neutrophil biology. Both basic scientists and clinicians will find a collection of this caliber to be an invaluable aid in their work with neutrophils.


Features

• Provides neutrophil protocols that are broadly applicable to leukocyte biology

• Includes protocols for isolation of neutrophils from humans and animal species

• Presents an interesting and informative overview of the field of neutrophil biology

• Facilitates a better understanding of the role of neutrophils in host defense


Readership

Molecular and Cell Biologists, Immunologists, Infectious Disease Specialists, Microbiologists

Buy this book

eBook n/a
  • ISBN 978-1-07-160154-9
  • Digitally watermarked, DRM-free
  • Included format:
  • ebooks can be used on all reading devices
Hardcover n/a
  • ISBN 978-1-07-160153-2
  • with online files
  • Free shipping for individuals worldwide
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Bibliographic Information

Bibliographic Information
Book Title
Neutrophil
Book Subtitle
Methods and Protocols
Editors
  • Mark T. Quinn
  • Frank DeLeo
Series Title
Methods in Molecular Biology
Series Volume
2087
Copyright
2020
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-07-160154-9
DOI
10.1007/978-1-0716-0154-9
Hardcover ISBN
978-1-07-160153-2
Series ISSN
1064-3745
Edition Number
3
Number of Pages
XX, 486
Number of Illustrations
35 b/w illustrations, 51 illustrations in colour
Topics