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Bioluminescent Imaging

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Steven Ripp

    1. The Center for Environmental Biotechnology, The University of Tennessee, 490 BioTech Inc., Knoxville, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step details essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2081)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
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  2. In Vitro/Ex Vivo Imaging Applications

    1. Front Matter

      Pages 1-1
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    2. High-Throughput Bioluminescence Imaging and Reporter Gene Assay with 3D Spheroids from Human Cell Lines

      • Maria Maddalena Calabretta, Laura Montali, Antonia Lopreside, Elisa Michelini, Aldo Roda
      Pages 3-14

    3. Bioluminescence Resonance Energy Transfer (BRET) Coupled Near-Infrared Imaging of Apoptotic Cells

      • Setsuko Tsuboi, Takashi Jin
      Pages 15-27

    4. High-Throughput Analysis of Endocrine-Disrupting Compounds Using BLYES and BLYAS Bioluminescent Yeast Bioassays

      • Tingting Xu, Anna Young, Jasleen Narula, Gary Sayler, Steven Ripp
      Pages 29-41

    5. Bioluminescent Imaging of Single Bacterial Cells Using an Enhanced ilux Operon

      • Carola Gregor
      Pages 43-52

    6. Bioluminescent Imaging and Tracking of Bacterial Transport in Soils

      • Jie Zhuang, Weipeng Liu, Liqiong Yang, Jia Kang, Xiaoming Zhang
      Pages 53-65

  3. In Vivo Imaging of Microbial Pathogenesis

    1. Front Matter

      Pages 67-67
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    2. In Vivo Bioluminescent Imaging of Yersinia ruckeri Pathogenesis in Fish

      • José A. Guijarro, Jessica Méndez
      Pages 69-80

    3. In Vivo Bioluminescent Monitoring of Parasites in BALB/c Mouse Models of Cutaneous Leishmaniasis Drug Discovery

      • Diana Caridha, Susan Leed, Alicia Cawlfield
      Pages 81-106

    4. Multiplex Imaging of Polymicrobial Communities—Murine Models to Study Oral Microbiome Interactions

      • Jens Kreth, Yasser M. Abdelrahman, Justin Merritt
      Pages 107-126

    5. Bioluminescence Imaging to Study Mature Biofilm Formation by Candida spp. and Antifungal Activity In Vitro and In Vivo

      • Katrien Van Dyck, Patrick Van Dijck, Greetje Vande Velde
      Pages 127-143

  4. In Vivo Small Animal/Organism Imaging Applications

    1. Front Matter

      Pages 145-145
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    2. Bioluminescence Imaging of Neuroinflammation in a Mouse Model of Parkinson’s Disease

      • Maria Eugenia Bernis, Gültekin Tamgüney
      Pages 147-159

    3. Continual Conscious Bioluminescent Imaging in Freely Moving Mice

      • Juan Antinao Diaz, Amy Geard, Lorna M. FitzPatrick, Juliette M. K. M. Delhove, Suzanne M. K. Buckley, Simon N. Waddington et al.
      Pages 161-175

    4. In Vivo Bioluminescent Imaging of Marburg Virus in a Rodent Model

      • Shan Lei, Weijin Huang, Youchun Wang, Qiang Liu
      Pages 177-190

    5. Continuous and Real-Time In Vivo Autobioluminescent Imaging in a Mouse Model

      • Derek Yip, Andrew Kirkpatrick, Tingting Xu, Tom Masi, Stacy Stephenson, Steven Ripp et al.
      Pages 191-201

    6. In Vivo Tracking of Tumor-Derived Bioluminescent Extracellular Vesicles in Mice

      • Prakash Gangadaran, Byeong-Cheol Ahn
      Pages 203-210

    7. Bioluminescence Imaging in the Chick Chorioallantoic Membrane Assay

      • Benedict Jefferies, Zhichao Tong, Roman Nawroth
      Pages 211-217

    8. Application of a cybLuc Aminoluciferin for Deep Tissue Bioluminescence Imaging in Rodent Models

      • Xiang Li, Minyong Li
      Pages 219-228
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Keywords

About this book

This volume provides detailed protocols and methodologies required to perform bioluminescent imaging in multiple stages, enabling the reader to integrate this technology into their laboratory-based imaging experiments. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Bioluminescent Imaging: Methods and Protocols to ensure successful results in the further study of this vital field.


Editors and Affiliations

  • The Center for Environmental Biotechnology, The University of Tennessee, 490 BioTech Inc., Knoxville, USA

    Steven Ripp

About the editor

.

Bibliographic Information

  • Book Title: Bioluminescent Imaging

  • Book Subtitle: Methods and Protocols

  • Editors: Steven Ripp

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9940-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-4939-9939-2Published: 13 November 2019

  • Softcover ISBN: 978-1-4939-9942-2Published: 13 November 2020

  • eBook ISBN: 978-1-4939-9940-8Published: 12 November 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 246

  • Number of Illustrations: 21 b/w illustrations, 63 illustrations in colour

  • Topics: Imaging / Radiology, Biotechnology

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