Genotype Phenotype Coupling
Methods and Protocols
Editors: Zielonka, Stefan, Krah, Simon (Eds.)
Free Preview- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes an implementation advice from the experts
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- About this book
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This volume aims at providing state-of-the-art protocols detailing ribosome display, cDNA display, phage display, yeast surface display, and mammalian display. Chapters guide readers through methods and protocols on in vitro methods over prokaryotic display systems, lower eukaryotes, and mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of all necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Genotype Phenotype Coupling aims to provide an overview of current technologies in this exciting and continuously evolving field.
- Table of contents (23 chapters)
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Screening, Post-SELEX Optimization and Application of DNA Aptamers Specific for Tobramycin
Pages 1-18
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Affitins: Ribosome Display for Selection of Aho7c-Based Affinity Proteins
Pages 19-41
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cDNA Display: A Stable and Simple Genotype–Phenotype Coupling Using a Cell-Free Translation System
Pages 43-56
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cDNA Display of Disulfide-Containing Peptide Library and In Vitro Evolution
Pages 57-77
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Rapid Antigen and Antibody-Like Molecule Discovery by Staphylococcal Surface Display
Pages 79-94
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Table of contents (23 chapters)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Genotype Phenotype Coupling
- Book Subtitle
- Methods and Protocols
- Editors
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- Stefan Zielonka
- Simon Krah
- Series Title
- Methods in Molecular Biology
- Series Volume
- 2070
- Copyright
- 2020
- Publisher
- Springer US
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-9853-1
- DOI
- 10.1007/978-1-4939-9853-1
- Hardcover ISBN
- 978-1-4939-9852-4
- Softcover ISBN
- 978-1-4939-9855-5
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XII, 445
- Number of Illustrations
- 25 b/w illustrations, 61 illustrations in colour
- Topics
