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Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols

Cutting-Edge Technologies and Advancements

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2069)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
  2. Rapid Methods for Detection of MRSA in Clinical Specimens

    • Elizabeth L. Palavecino
    Pages 29-45
  3. Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSA

    • Tetsuo Yamaguchi, Daisuke Ono, Ayami Sato
    Pages 59-78
  4. Multilocus Sequence Typing of Staphylococcus aureus

    • Ying Liu, Yinduo Ji
    Pages 95-102
  5. Genetic Manipulations of Staphylococcal Chromosomal DNA

    • Crystal M. Austin, Jeffrey L. Bose
    Pages 103-111
  6. Genetic Manipulation of MRSA Using CRISPR/Cas9 Technology

    • Weizhong Chen, Quanjiang Ji
    Pages 113-124
  7. Identification of Virulence Determinants During Host-Pathogen Interaction Using Tn-Seq Technology

    • Christopher T. Peek, Carolyn B. Ibberson, James E. Cassat
    Pages 155-175
  8. Metabolomic Profiling of Staphylococcus aureus

    • Ting Lei, Qingqing Mao, Chi Chen, Yinduo Ji
    Pages 177-186
  9. Preclinical Models and Methodologies for Monitoring Staphylococcus aureus Infections Using Noninvasive Optical Imaging

    • Nathan K. Archer, Yu Wang, Roger V. Ortines, Haiyun Liu, Sabrina J. Nolan, Qi Liu et al.
    Pages 197-228
  10. Animal Models for Drug Development for MRSA

    • Andrea Marra
    Pages 253-266
  11. Back Matter

    Pages 267-268

About this book

This third edition volume expands on the previous editions with an update on the latest techniques used for the detection, genotyping, and investigating pathogenesis of Staphylococcus aureus in vitro and in vivo. The methods covered in this book mostly focus on routine clinical diagnosis, surveillance, research, and practice for treatment of patients infected by multi-drug resistant S. aureus. The book also covers the epidemiology of MRSA, molecular typing approaches, clinical treatment of MRSA infections, and animal models of drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Informative and cutting-edge, Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols: Cutting-Edge Technologies and Advancements, Third Edition is a valuable resource for researchers looking to set up new methods to study S. aureus, and will also be very useful for technicians and scientists working on other bacterial pathogens. 


Editors and Affiliations

  • Department of Veterinary Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Saint Paul, USA

    Yinduo Ji

Bibliographic Information

  • Book Title: Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols

  • Book Subtitle: Cutting-Edge Technologies and Advancements

  • Editors: Yinduo Ji

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9849-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-4939-9848-7Published: 16 September 2019

  • Softcover ISBN: 978-1-4939-9851-7Published: 16 September 2020

  • eBook ISBN: 978-1-4939-9849-4Published: 15 September 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XI, 268

  • Number of Illustrations: 8 b/w illustrations, 27 illustrations in colour

  • Topics: Bacteriology, Infectious Diseases

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access