The Eukaryotic RNA Exosome
Methods and Protocols
Editors: LaCava, John, Vaňáčová, Štěpánka (Eds.)
Free Preview- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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This volume provides a cross-section of RNA exosome research protocols, applied to a diversity of model organisms. Chapters guide readers through methods that e.g. delineate eukaryotic exosomes’ origins in prokaryotes, probe its RNA substrates, adapter complexes and macromolecular interaction of networks, and establish critical structural-function relationships. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, The Eukaryotic RNA Exosome: Methods and Protocols aims to ensure successful results in the further study of this vital field.
- About the authors
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- Table of contents (25 chapters)
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The RNA Exosome and Human Disease
Pages 3-33
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The Bacterial Counterparts of the Eukaryotic Exosome: An Evolutionary Perspective
Pages 37-46
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In Vitro Characterization of the Prokaryotic Counterparts of the Exosome Complex
Pages 47-61
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Enzymatic Analysis of Reconstituted Archaeal Exosomes
Pages 63-79
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Protocols for Northern Analysis of Exosome Substrates and Other Noncoding RNAs
Pages 83-103
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Table of contents (25 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- The Eukaryotic RNA Exosome
- Book Subtitle
- Methods and Protocols
- Editors
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- John LaCava
- Štěpánka Vaňáčová
- Series Title
- Methods in Molecular Biology
- Series Volume
- 2062
- Copyright
- 2020
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-9822-7
- DOI
- 10.1007/978-1-4939-9822-7
- Hardcover ISBN
- 978-1-4939-9821-0
- Softcover ISBN
- 978-1-4939-9824-1
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XIII, 517
- Number of Illustrations
- 27 b/w illustrations, 52 illustrations in colour
- Topics
