- Includes cutting-edge techniques
- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
Buy this book
- About this book
-
This book provides a thorough introduction to widely used techniques for the study of the intersection between developmental biology and neuroscience, an exceptional area to address and investigate impacting biological questions. The fully updated volume examines cutting-edge techniques on a representative range of animals, including widely used genetic model systems, such as the fruit fly, zebra fish, chicken, and mouse, as well as non-canonical experimental systems opened up through the advent of genome editing. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Brain Development: Methods and Protocols, Second Edition is an ideal guide for researchers interested in utilizing recent technical advances in molecular genetics for the study of the brain.
- Table of contents (29 chapters)
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Combining BrdU-Labeling to Detection of Neuronal Markers to Monitor Adult Neurogenesis in Hydra
Pages 3-24
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Reverse Genetic Approaches to Investigate the Neurobiology of the Cnidarian Sea Anemone Nematostella vectensis
Pages 25-43
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Generating Transgenic Reporter Lines for Studying Nervous System Development in the Cnidarian Nematostella vectensis
Pages 45-57
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Immunostaining and In Situ Hybridization of the Developing Acoel Nervous System
Pages 59-80
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Immunostaining of the Embryonic and Larval Drosophila Brain
Pages 81-96
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Table of contents (29 chapters)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Brain Development
- Book Subtitle
- Methods and Protocols
- Editors
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- Simon G. Sprecher
- Series Title
- Methods in Molecular Biology
- Series Volume
- 2047
- Copyright
- 2020
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-9732-9
- DOI
- 10.1007/978-1-4939-9732-9
- Hardcover ISBN
- 978-1-4939-9731-2
- Softcover ISBN
- 978-1-4939-9734-3
- Series ISSN
- 1064-3745
- Edition Number
- 2
- Number of Pages
- XIV, 527
- Number of Illustrations
- 20 b/w illustrations, 96 illustrations in colour
- Topics