Experimental Cholestasis Research

1. Front Matter

   Pages i-xiii

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2. Mechanisms of Drug-Induced Cholestasis

   • Eva Gijbels, Mathieu Vinken

   Pages 1-14

3. LC-MS/MS Analysis of Bile Acids in In Vitro Samples

   • Debbie Dewaele, Pieter Annaert, Eef Hoeben

   Pages 15-23

4. Pipe-3D: A Pipeline Based on Immunofluorescence, 3D Confocal Imaging, Reconstructions, and Morphometry for Biliary Network Analysis in Cholestasis

   • Amruta Damle-Vartak, Brigitte Begher-Tibbe, Georgia Gunther, Fabian Geisler, Nachiket Vartak, Jan G. Hengstler

   Pages 25-53

5. Vesicle- and Hepatocyte-Based Assays for Identification of Drug Candidates Inhibiting BSEP Function

   • Pieter Van Brantegem, Neel Deferm, Bing Qi, Tom De Vocht, Pieter Annaert

   Pages 55-73

6. The C-DILI™ Assay: An Integrated In Vitro Approach to Predict Cholestatic Hepatotoxicity

   • Jonathan P. Jackson, Kenneth R. Brouwer

   Pages 75-85

7. In Vivo Measurement of Hepatic Drug Transporter Inhibition with Radiolabeled Bile Acids

   • Stef De Lombaerde, Sara Neyt, Christian Vanhove, Filip De Vos

   Pages 87-98

8. Measuring the Impact of Bile Acids on the Membrane Order of Primary Hepatocytes and Isolated Mitochondria by Fluorescence Imaging and Spectroscopy

   • Tânia Sousa, Rui E. Castro, Ana Coutinho, Cecília M. P. Rodrigues, Manuel Prieto, Fábio Fernandes

   Pages 99-115

9. The Role and Study of Mitochondrial Impairment and Oxidative Stress in Cholestasis

   • Reza Heidari, Hossein Niknahad

   Pages 117-132

10. Measuring Apoptosis and Necrosis in Cholestatic Liver Injury

    • Benjamin L. Woolbright, Hartmut Jaeschke

    Pages 133-147

11. Necrosome Formation and Necroptosis in Experimental Cholestasis

    • Marta B. Afonso, Cecília M. P. Rodrigues

    Pages 149-162

12. Proteomics in Primary Biliary Cholangitis

    • You Li, Ruqui Tang, Patrick S. C. Leung, M. Eric Gershwin, Xiong Ma

    Pages 163-173

13. Transcriptomic Analysis of Cholestatic Compounds In Vitro

    • Céline Parmentier, Philippe Couttet, Marianne Uteng, Armin Wolf, Lysiane Richert

    Pages 175-186

14. System Microscopy of Stress Response Pathways in Cholestasis Research

    • Johannes P. Schimming, Bas ter Braak, Marije Niemeijer, Steven Wink, Bob van de Water

    Pages 187-202

15. Mouse Models for Diseases in the Cholangiocyte Lineage

    • Noémi Van Hul, Urban Lendahl, Emma R. Andersson

    Pages 203-236

16. Common Bile Duct Ligation as Model for Secondary Biliary Cirrhosis

    • Sanne Van Campenhout, Hans Van Vlierberghe, Lindsey Devisscher

    Pages 237-247

17. 3,5-Diethoxycarbonyl-1,4-Dihydrocollidine Diet: A Rodent Model in Cholestasis Research

    • Elisa Pose, Pau Sancho-Bru, Mar Coll

    Pages 249-257

18. A Rotavirus-Induced Mouse Model to Study Biliary Atresia and Neonatal Cholestasis

    • Sujit K. Mohanty, Bryan Donnelly, Haley Temple, Gregory M. Tiao

    Pages 259-271

19. Zebrafish as a Model to Study Cholestatic Liver Diseases

    • Duc-Hung Pham, Chunyue Yin

    Pages 273-289

20. Setup and Use of HepaRG Cells in Cholestasis Research

    • Christiane Guguen-Guillouzo, André Guillouzo

    Pages 291-312

This detailed book provides a state-of-the-art compilation of protocols relevant for the experimental study of cholestasis, each written by internationally recognized research teams that have built up long-standing expertise regarding one or more aspects of experimental cholestasis research. The first half of the collection examines methods targeted towards studying established and new mechanisms of cholestasis, while the second half explores experimental models and tools to investigate mechanisms of cholestasis and/or to predict cholestatic properties of drugs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Experimental Cholestasis Research serves as an ideal guide for basic andapplied researchers pursuing this vital area of research in pharmacology and toxicology.

• Drug-induced liver injury
• Liver toxicology
• DILI
• Cholestatic drug properties
• Model organisms
• Impaired bile secretion
• Hepatocellular injury
• hepatology

• Department of In Vitro Toxicology and Dermato-Cosmetology, Vrije Universiteit Brussel, Brussels, Belgium

  Mathieu Vinken

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