Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1905)
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Table of contents (21 protocols)
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Front Matter
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Isolation of Progenitor Cells
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Front Matter
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Characterization of Liver Progenitors In Vivo
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Generation of Hepatocytes, Cholangiocytes, and Their Progenitors
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Front Matter
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Reconstitution of Liver Tissue Structures
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Front Matter
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Keywords
About this book
This volume looks at the liver’s epithelial cells—hepatocytes and cholangiocytes—and their progenitors. This book is divided into five parts: isolation of progenitor cells; characterization of liver progenitors in vivo; generation of hepatocytes, cholangiocytes, and their progenitors; reconstitution of liver tissue structures; and liver injury models. The chapters in this book cover topics such as expansion of bipotential liver stem/progenitor cells (LPCs) from fetal and neonatal liver; identifying progenitor cells involved in liver regeneration in vivo; methods for generating hepatocytes and cholangiocytes from multiple cellular sources; 3D tissue structures ex vivo; and resolving hepatic fibrosis by bone marrow transplantation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Hepatic Stem Cells: Methods and Protocols is a valuable resource to help researchers understand the current theories used to study hepatic stem/progenitor cells, and aid them in performing experiments related to liver biology and pathophysiology.
Editors and Affiliations
Bibliographic Information
Book Title: Hepatic Stem Cells
Book Subtitle: Methods and Protocols
Editors: Naoki Tanimizu
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-8961-4
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019
Hardcover ISBN: 978-1-4939-8960-7Published: 08 December 2018
eBook ISBN: 978-1-4939-8961-4Published: 08 December 2018
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 248
Number of Illustrations: 11 b/w illustrations, 59 illustrations in colour
Topics: Stem Cells