Skip to main content
SpringerLink
Log in
Book cover

Microinjection

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Chengyu Liu

    1. National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Yubin Du

    1. National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques for the study of microinjection and its purposes
  • Focuses on mouse models for half of the book, with the other half centered on a wide variety of other models
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1874)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (30 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Microinjection and Micromanipulation: A Historical Perspective

    • Wenhao Xu
    Pages 1-16

  3. Production of Transgenic Mice by Pronuclear Microinjection

    • Xin-an Pu, Anthony P. Young, H. Michael Kubisch
    Pages 17-41

  4. Transgene Recombineering in Bacterial Artificial Chromosomes

    • Michael G. Zeidler, Thomas L. Saunders
    Pages 43-69

  5. Using TARGATT™ Technology to Generate Site-Specific Transgenic Mice

    • Ruby Yanru Chen-Tsai
    Pages 71-86

  6. Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated Method

    • Takuya Sato, Takehiko Ogawa
    Pages 87-98

  7. Chimeric Mouse Generation by ES Cell Blastocyst Microinjection and Uterine Transfer

    • Yubin Du, Wen Xie, Fan Zhang, Chengyu Liu
    Pages 99-114

  8. Creating Knockin Alleles in Mouse Embryonic Stem Cells by CRISPR/Cas9-Mediated Homologous Recombination Without Drug Selection

    • Pinghu Liu, Yan Li, Jingqi Lei, Lijin Dong
    Pages 115-137

  9. Using CRISPR/Cas9 for Gene Knockout in Immunodeficient NSG Mice

    • Yubin Du, Wen Xie, Fan Zhang, Uimook Choi, Chengyu Liu, Colin L. Sweeney
    Pages 139-168

  10. Generation of CRISPR-Edited Rodents Using a Piezo-Driven Zygote Injection Technique

    • Melissa A. Scott, Yueh-Chiang Hu
    Pages 169-178

  11. Delivery of CRISPR-Cas9 into Mouse Zygotes by Electroporation

    • Wenning Qin, Haoyi Wang
    Pages 179-190

  12. Generation of Conditional Knockout Mice by Sequential Insertion of Two loxP Sites In Cis Using CRISPR/Cas9 and Single-Stranded DNA Oligonucleotides

    • Ye Liu, Yubin Du, Wen Xie, Fan Zhang, Douglas Forrest, Chengyu Liu
    Pages 191-210

  13. Improvement of Mouse Cloning from Any Type of Cell by Nuclear Injection

    • Sayaka Wakayama, Satoshi Kishigami, Teruhiko Wakayama
    Pages 211-228

  14. The CARD Method for Simple Vitrification of Mouse Oocytes: Advantages and Applications

    • Naomi Nakagata, Jorge Sztein, Toru Takeo
    Pages 229-242

  15. The CARD Method for Mouse Sperm Cryopreservation and In Vitro Fertilization Using Frozen-Thawed Sperm

    • Toru Takeo, Jorge Sztein, Naomi Nakagata
    Pages 243-256

  16. Isolation and Analysis of a Genome-Edited Single-Hepatocyte from a Cas9 Transgenic Mouse Line

    • Takayuki Sakurai, Akiko Kamiyoshi, Masato Ohtsuka, Channabasavaiah B. Gurumurthy, Masahiro Sato, Takayuki Shindo
    Pages 257-271

  17. Microinjection and Oviduct Transfer Procedures for Rat Model Generation with CRISPR-Cas9 Technology

    • Tuija M. Alcantar, Xin Y. Rairdan
    Pages 273-294

  18. Molecular Aspects of Zinc Finger Nucleases (ZFNs)-Mediated Gene Editing in Rat Embryos

    • Xiaoxia Cui
    Pages 295-312

  19. Organ Generation from Knockedout Rat Blastocysts Complemented with Pluripotent Stem Cells

    • Masumi Hirabayashi, Shinichi Hochi
    Pages 313-326

  20. Generation of Rabbit Models by Gene Editing Nucleases

    • Dongshan Yang, Jie Xu, Y. Eugene Chen
    Pages 327-345
Back to top

Keywords

About this book

This detailed book explores how microinjection will be used in the foreseeable future, not only for generating animal models for biomedical research but also for changing economically or ecologically important species that can broadly impact our society in general. The opening half of the book focuses on methods for generating mouse models, as they are still the most popular in genome engineering research, while the second half examines gene-editing in a variety of other species, opened up by the developments in ZFN, TALEN, and CRISPR techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Microinjection: Methods and Protocols serves as an ideal guide for researchers looking to take advantage of the breakthrough technologies in gene-editing and embryo micromanipulations.

Editors and Affiliations

  • National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, USA

    Chengyu Liu, Yubin Du

Bibliographic Information

  • Book Title: Microinjection

  • Book Subtitle: Methods and Protocols

  • Editors: Chengyu Liu, Yubin Du

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8831-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8830-3Published: 24 October 2018

  • Softcover ISBN: 978-1-4939-9398-7Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8831-0Published: 23 October 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 540

  • Number of Illustrations: 38 b/w illustrations, 96 illustrations in colour

  • Topics: Biotechnology, Human Genetics

Publish with us

Policies and ethics

Back to top

Search

Navigation