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Methods in Molecular Biology

Heterologous Expression of Membrane Proteins

Methods and Protocols

Editors: Mus-Veteau, Isabelle (Ed.)

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  • Includes cutting-edge methods and protocols for the study of recombinant integral membrane proteins
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook 83,29 €
price for France (gross)
  • ISBN 978-1-4939-3637-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 147,69 €
price for France (gross)
  • ISBN 978-1-4939-3635-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover 105,49 €
price for France (gross)
  • ISBN 978-1-4939-8102-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This detailed volume encompasses chapters from leading experts in the area of membrane proteins who describe step-by-step protocols developed these last few years to improve the functional production and stabilization of recombinant integral membrane proteins (IMPs). Membrane proteins play a key role in numerous pathologies such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and Alzheimer’s disease, yet studies on these and other disorders are hampered by a lack of information about the proteins involved. This book sets out to aid researchers in rectifying this situation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Heterologous Expression of Membrane Proteins: Methods and Protocols, Second Edition serves as an ideal guide for scientists attempting to delve deeper into the myriad unique IMP structures.

Table of contents (19 chapters)

Table of contents (19 chapters)
  • Cell-Free Production of Membrane Proteins in Escherichia coli Lysates for Functional and Structural Studies

    Pages 1-21

    Rues, Ralf-Bernhardt (et al.)

  • Membrane Protein Production in the Yeast, S. cerevisiae

    Pages 23-35

    Cartwright, Stephanie P. (et al.)

  • Membrane Protein Production in Escherichia coli: Protocols and Rules

    Pages 37-52

    Angius, Federica (et al.)

  • Codon Optimizing for Increased Membrane Protein Production: A Minimalist Approach

    Pages 53-61

    Mirzadeh, Kiavash (et al.)

  • Generation of Tetracycline-Inducible Mammalian Cell Lines by Flow Cytometry for Improved Overproduction of Membrane Proteins

    Pages 63-78

    Andréll, Juni (et al.)

Buy this book

eBook 83,29 €
price for France (gross)
  • ISBN 978-1-4939-3637-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 147,69 €
price for France (gross)
  • ISBN 978-1-4939-3635-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover 105,49 €
price for France (gross)
  • ISBN 978-1-4939-8102-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Heterologous Expression of Membrane Proteins
Book Subtitle
Methods and Protocols
Editors
  • Isabelle Mus-Veteau
Series Title
Methods in Molecular Biology
Series Volume
1432
Copyright
2016
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-3637-3
DOI
10.1007/978-1-4939-3637-3
Hardcover ISBN
978-1-4939-3635-9
Softcover ISBN
978-1-4939-8102-1
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XII, 322
Number of Illustrations
27 b/w illustrations, 39 illustrations in colour
Topics