Overview
- Presents current up-to-date methodologies
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1136)
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Table of contents (14 protocols)
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Front Matter
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Imaging and Measurement of Mitotic Spindle Dynamics In Vivo
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Front Matter
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Analysis and Manipulation of the Microtubule Cytoskeleton in Cells and In Vitro
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Front Matter
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Specialized Biophysical and Microscopy Approaches for Analyzing Microtubules and Microtubule Associated Proteins
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Front Matter
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Back Matter
Keywords
- cell division
- chromosome segregation
- complex cell free extracts
- microtubule based machine
- microtubule cytoskeleton in cells
- microtubule structure in cells
- microtubule-associated proteins
- microtubules
- mitosis
- mitotic defects
- quantifying aneuploidy
- spindle apparatus
- Cell Cycle Analysis
- gene expression
About this book
Mitosis: Methods and Protocols provides state-of-the-art overviews on the most important approaches currently used in mitosis research spanning from the analysis of single molecules in isolation to their utilization within the complex environment of the cell. The volume is divided into four parts, each focused on methods pertaining to distinct aspects of mitosis research. Part I presents approaches for visualizing and analyzing the dynamic behaviors of the spindle apparatus, the microtubule based machine that drives chromosome segregation. Part II focuses more generally on methods for studying and manipulating the microtubule cytoskeleton in cells and complex cell free extracts. Part III provides state of the art biophysical and high resolution microscopy approaches for assessing complex interactions between microtubules and microtubule-associated proteins in isolation as well as microtubule structure in cells. Part IV provides methods for studying the effects of cell shape on cell division and methods for quantifying aneuploidy (aberrant chromosome number) which frequently results from mitotic defects and has been linked to human maladies ranging from birth defects to cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Mitosis: Methods and Protocols seeks to provide diverse methods and new techniques to address new or old questions related to the mechanisms of mitosis.
Editors and Affiliations
Bibliographic Information
Book Title: Mitosis
Book Subtitle: Methods and Protocols
Editors: David J. Sharp
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-0329-0
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, New York 2014
Hardcover ISBN: 978-1-4939-0328-3Published: 15 March 2014
Softcover ISBN: 978-1-4939-4301-2Published: 23 August 2016
eBook ISBN: 978-1-4939-0329-0Published: 14 March 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 308
Number of Illustrations: 12 b/w illustrations, 71 illustrations in colour
Topics: Cell Biology, Cell Cycle Analysis, Gene Expression