- Presents current up-to-date methodologies
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Buy this book
- About this book
-
Mitosis: Methods and Protocols provides state-of-the-art overviews on the most important approaches currently used in mitosis research spanning from the analysis of single molecules in isolation to their utilization within the complex environment of the cell. The volume is divided into four parts, each focused on methods pertaining to distinct aspects of mitosis research. Part I presents approaches for visualizing and analyzing the dynamic behaviors of the spindle apparatus, the microtubule based machine that drives chromosome segregation. Part II focuses more generally on methods for studying and manipulating the microtubule cytoskeleton in cells and complex cell free extracts. Part III provides state of the art biophysical and high resolution microscopy approaches for assessing complex interactions between microtubules and microtubule-associated proteins in isolation as well as microtubule structure in cells. Part IV provides methods for studying the effects of cell shape on cell division and methods for quantifying aneuploidy (aberrant chromosome number) which frequently results from mitotic defects and has been linked to human maladies ranging from birth defects to cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Mitosis: Methods and Protocols seeks to provide diverse methods and new techniques to address new or old questions related to the mechanisms of mitosis.
- Table of contents (14 chapters)
-
-
Analysis of Mitotic Protein Dynamics and Function in Drosophila Embryos by Live Cell Imaging and Quantitative Modeling
Pages 3-30
-
Rapid Measurement of Mitotic Spindle Orientation in Cultured Mammalian Cells
Pages 31-40
-
Automated Segmentation of the First Mitotic Spindle in Differential Interference Contrast Microcopy Images of C. elegans Embryos
Pages 41-45
-
Imaging the Mitotic Spindle by Spinning Disk Microscopy in Tobacco Suspension Cultured Cells
Pages 47-55
-
Image-Based Computational Tracking and Analysis of Spindle Protein Dynamics
Pages 57-78
-
Table of contents (14 chapters)
- Download Preface 1 PDF (31.1 KB)
- Download Sample pages 1 PDF (154.6 KB)
- Download Table of contents PDF (53 KB)
Recommended for you
Bibliographic Information
- Bibliographic Information
-
- Book Title
- Mitosis
- Book Subtitle
- Methods and Protocols
- Editors
-
- David J. Sharp
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1136
- Copyright
- 2014
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, New York
- eBook ISBN
- 978-1-4939-0329-0
- DOI
- 10.1007/978-1-4939-0329-0
- Hardcover ISBN
- 978-1-4939-0328-3
- Softcover ISBN
- 978-1-4939-4301-2
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- X, 308
- Number of Illustrations
- 12 b/w illustrations, 71 illustrations in colour
- Topics
