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Chloroplast Biotechnology

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Pal Maliga

    1. Waksman Institute of Microbiology, Rutgers University, Piscataway, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2317)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xii
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  2. Background

    1. Front Matter

      Pages 1-1
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    2. Plastid Genomes of Flowering Plants: Essential Principles

      • Tracey A. Ruhlman, Robert K. Jansen
      Pages 3-47

    3. Plastid Gene Transcription: An Update on Promoters and RNA Polymerases

      • Jennifer Ortelt, Gerhard Link
      Pages 49-76

    4. Engineering Chloroplasts for High-Level Constitutive or Inducible Transgene Expression

      • Ralph Bock
      Pages 77-94

    5. Marker-Free Transplastomic Plants by Excision of Plastid Marker Genes Using Directly Repeated DNA Sequences

      • Elisabeth A. Mudd, Panagiotis Madesis, Elena Martin Avila, Anil Day
      Pages 95-107

    6. Fluorescent Labeling and Confocal Microcopy of Plastids and Stromules

      • Maureen R. Hanson, Patricia L. Conklin, Amirali Sattarzadeh
      Pages 109-132

  3. Nicotiana Tabacum: The Model Species of Chloroplast Biotechnology

    1. Front Matter

      Pages 133-133
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    2. Transformation of the Plastid Genome in Tobacco: The Model System for Chloroplast Genome Engineering

      • Pal Maliga, Tarinee Tungsuchat-Huang, Kerry Ann Lutz
      Pages 135-153

    3. Nicotiana tabacum: An Update on PEG-Mediated Plastid Transformation

      • Areli Herrera Díaz, Hans-Ulrich Koop
      Pages 155-166

    4. Transformation of the Plastid Genome in Tobacco Suspension Cell Cultures

      • Jeffrey M. Staub
      Pages 167-175

    5. Plastid Marker Gene Excision in the Tobacco Shoot Apex by Agrobacterium-Delivered Cre Recombinase

      • Tarinee Tungsuchat-Huang, Pal Maliga
      Pages 177-193

    6. Rubisco Engineering by Plastid Transformation and Protocols for Assessing Expression

      • Spencer M. Whitney, Robert E. Sharwood
      Pages 195-214

  4. Crop Specific Plastid Transformation Protocols

    1. Front Matter

      Pages 215-215
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    2. Plastid Transformation in Tomato: A Vegetable Crop and Model Species

      • Stephanie Ruf, Ralph Bock
      Pages 217-228

    3. Stable Plastid Transformation of Petunia for Studies in Basic Research

      • Elena Martin Avila, Anil Day
      Pages 229-245

    4. Plastid Transformation in Potato: An Important Source of Nutrition and Industrial Materials

      • Vladimir T. Valkov, Daniela Gargano, Teodoro Cardi, Nunzia Scotti
      Pages 247-256

    5. Plastid Transformation in Poplar: A Model for Perennial Trees

      • Yuyong Wu, Ling Chang, Chunmei Jiang, Letian Xu, Jiang Zhang
      Pages 257-265

    6. Biolistic Plastid Transformation in Lettuce (Lactuca sativa) for Oral Delivery of Biopharmaceuticals

      • Tracey A. Ruhlman
      Pages 267-281

    7. Plastid Transformation in Sugar Beet: An Important Industrial Crop

      • Francesca De Marchis, Michele Bellucci
      Pages 283-290
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Keywords

About this book

This second edition volume expands on the previous edition with updated chapters in model systems and new crops. The book contains protocols for plastid engineering in leaves, tissue culture cells, and the shoot apex of plants, as well as for marker excision from the plastid genome and engineering Rubisco, the key enzyme of photosynthesis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 


Cutting-edge and practical, Chloroplast Biotechnology: Methods and Protocols, Second Edition is a valuable resource for researchers who wish to enter the field, and for practitioners looking for insights 
of applications in agriculture, industrial biotechnology and healthcare.  





Editors and Affiliations

  • Waksman Institute of Microbiology, Rutgers University, Piscataway, USA

    Pal Maliga

Bibliographic Information

  • Book Title: Chloroplast Biotechnology

  • Book Subtitle: Methods and Protocols

  • Editors: Pal Maliga

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1472-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1471-6Published: 25 May 2021

  • Softcover ISBN: 978-1-0716-1474-7Published: 24 May 2021

  • eBook ISBN: 978-1-0716-1472-3Published: 24 May 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 369

  • Number of Illustrations: 29 b/w illustrations, 41 illustrations in colour

  • Topics: Biotechnology, Plant Sciences, Plant Breeding/Biotechnology

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