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Doubled Haploid Technology

Volume 3: Emerging Tools, Cucurbits, Trees, Other Species

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains keynotes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2289)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
  2. Doubled Haploids in Cucurbits

    1. Front Matter

      Pages 69-69
    2. Doubled Haploid Parthenogenetic Production of Melon ‘Piel de Sapo’

      • Isidre d’Hooghvorst, Oscar Torrico, Salvador Nogués
      Pages 87-95
    3. Doubled Haploid Production in Watermelon

      • Nebahat Sari, Ilknur Solmaz
      Pages 97-110
    4. Anther Culture in Cucurbita Species

      • Ertan Sait Kurtar, Musa Seymen
      Pages 111-121
    5. Gynogenesis in Cucurbita Species

      • Ertan Sait Kurtar, Musa Seymen
      Pages 123-133
    6. Induction of Parthenogenesis by Irradiated Pollen in Cucurbita Species

      • Ertan Sait Kurtar, Musa Seymen
      Pages 135-145
  3. Doubled Haploids in Woody Species

    1. Front Matter

      Pages 147-147
    2. Microspore Embryogenesis in Citrus

      • Emna Yahyaoui, Maria Antonietta Germanà
      Pages 149-166
    3. Anther Culture in Coconut (Cocos nucifera L.)

      • P. I. Prasanthi Perera, Vijitha R. M. Vidhanaarachchi
      Pages 167-178
    4. Production of Haploid and Doubled Haploid Lines in Nut Crops: Persian Walnut, Almond, and Hazelnut

      • Kourosh Vahdati, Mohammad Sadat-Hosseini, Pedro Martínez-Gómez, Maria Antonietta Germanà
      Pages 179-198
    5. Production of Doubled Haploid Embryos from Cork Oak Anther Cultures by Antimitotic Agents and Temperature Stress

      • Beatriz Pintos, José Antonio Manzanera, Arancha Gómez-Garay
      Pages 199-219
    6. Anther Culture in Jatropha curcas L.: A Tree Species

      • Savarimuthu Arockiasamy, Manoj Patil, Vijay Yepuri, Vineeta Shrivastava, Neeta Madan, Sainath Hadole et al.
      Pages 221-233
  4. Doubled Haploids in Other Species

    1. Front Matter

      Pages 235-235
    2. Haploid Plant Production in Borage (Borago officinalis L.) by Anther Culture

      • Mohammad Reza Abdollahi, Zahra Chardoli Eshaghi, Jose M. Seguí-Simarro
      Pages 237-248

About this book

This title offers 62 chapters divided among three volumes covering the latest topics dealing with Doubled Haploid (DH) technology, as well as methods to produce DHs in different species through different in vivo and in vitro approaches. Volume 3 looks at the emerging tools used in DH technology; protocols based on anther culture, in vitro gynogenesis and hybridization with irradiated pollen for DH production in cucubits such as cucumber, melon, watermelon, and summer squash; techniques to produce haploids and DHs in different woody species and genera such as citrus, coconut, walnut, and almond; and methods for DH production in borage, Saintpaulia ionantha, cow cockle, and marigold. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and comprehensive, Doubled Haploid Technology, Volume 3: Emerging Tools, Cucurbits, Trees, Other Species is a valuable resource for scientists and researchers looking to learn more about this interesting and developing field.



Editors and Affiliations

  • Cell Biology Group — COMAV Institute, Universitat Politècnica de València, Valencia, Spain

    Jose M. Segui-Simarro

Bibliographic Information

  • Book Title: Doubled Haploid Technology

  • Book Subtitle: Volume 3: Emerging Tools, Cucurbits, Trees, Other Species

  • Editors: Jose M. Segui-Simarro

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1331-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1330-6Published: 17 July 2021

  • Softcover ISBN: 978-1-0716-1333-7Published: 18 July 2022

  • eBook ISBN: 978-1-0716-1331-3Published: 16 July 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 327

  • Number of Illustrations: 2 b/w illustrations, 64 illustrations in colour

  • Topics: Plant Sciences

Buy it now

Buying options

eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access