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Single Stranded DNA Binding Proteins

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2281)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xi
  2. The Essential, Ubiquitous Single-Stranded DNA-Binding Proteins

    • Marcos T. Oliveira, Grzegorz L. Ciesielski
    Pages 1-21
  3. Single-Stranded DNA-Binding Proteins in the Archaea

    • Najwa Taib, Simonetta Gribaldo, Stuart A. MacNeill
    Pages 23-47
  4. Single-Molecule Fluorescence Methods to Study Protein Exchange Kinetics in Supramolecular Complexes

    • Richard R. Spinks, Lisanne M. Spenkelink, Antoine M. van Oijen
    Pages 49-65
  5. Magnetic Tweezers-Based Single-Molecule Assays to Study Interaction of E. coli SSB with DNA and RecQ Helicase

    • Debjani Bagchi, Weiting Zhang, Samar Hodeib, Bertrand Ducos, Vincent Croquette, Maria Manosas
    Pages 93-115
  6. Generation of Fluorescent Versions of Saccharomyces cerevisiae RPA to Study the Conformational Dynamics of Its ssDNA-Binding Domains

    • Sahiti Kuppa, Nilisha Pokhrel, Elliot Corless, Sofia Origanti, Edwin Antony
    Pages 151-168
  7. Atomic Force Microscopy Reveals that the Drosophila Telomere-Capping Protein Verrocchio Is a Single-Stranded DNA-Binding Protein

    • Alessandro Cicconi, Emanuela Micheli, Grazia Daniela Raffa, Stefano Cacchione
    Pages 241-263
  8. Optical Tweezers to Investigate the Structure and Energetics of Single-Stranded DNA-Binding Protein-DNA Complexes

    • José A. Morin, Fernando Cerrón, Francisco J. Cao-García, Borja Ibarra
    Pages 273-288

About this book

This volume provides a comprehensive set of protocols that can be used by any research lab to investigate diverse functional and structural properties of Single Stranded DNA Binding Proteins (SSBs) from eubacterial, archaeal, eukaryotic, mitochondrial and viral systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Single Stranded DNA Binding Proteins aims to be a useful practical guide to researchers to help further their study in this field. 


Editors and Affiliations

  • Jaboticabal, Brazil

    Marcos T. Oliveira

Bibliographic Information

  • Book Title: Single Stranded DNA Binding Proteins

  • Editors: Marcos T. Oliveira

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1290-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1289-7Published: 14 April 2021

  • Softcover ISBN: 978-1-0716-1292-7Published: 15 April 2022

  • eBook ISBN: 978-1-0716-1290-3Published: 13 April 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 376

  • Number of Illustrations: 13 b/w illustrations, 64 illustrations in colour

  • Topics: Protein Science

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 149.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access