Methods in Molecular Biology

Next Generation Culture Platforms for Reliable In Vitro Models

Methods and Protocols

Editors: Brevini, Tiziana A.L., Fazeli, Alireza, Turksen, Kursad (Eds.)

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  • Includes cutting-edge techniques
  • Provides step-by-step details essential for reproducible results
  • Contains key implementation advice from the experts
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eBook 139,99 €
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  • ISBN 978-1-0716-1246-0
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Hardcover 179,34 €
price for France (gross)
  • ISBN 978-1-0716-1245-3
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About this book

This detailed book collects original protocols aimed at encouraging and stimulating the scientific community to design and produce models for the laboratory that mimic cell guidance conditions as they occur in vivo. The protocols collected describe powerful strategies to exploit chemical cues involved in cell differentiation processes. Special emphasis is given to the use of methods for purification and characterization of exosomes and other secreted vesicles, as well as micro and non-coding RNAs, that have been demonstrated to control the tuning of the in vivo micro and macro environment in order to ensure the optimal soluble environment in vitro. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Next Generation Culture Platforms for Reliable In Vitro Models: Methods and Protocols serves as an ideal guide for researchers working toward developing these vital testing models for preclinical studies.

Table of contents (20 chapters)

Table of contents (20 chapters)
  • Recreating the Follicular Environment: A Customized Approach for In Vitro Culture of Bovine Oocytes Based on the Origin and Differentiation State

    Pages 1-15

    Luciano, Alberto Maria (et al.)

  • Clinostat 3D Cell Culture: Protocols for the Preparation and Functional Analysis of Highly Reproducible, Large, Uniform Spheroids and Organoids

    Pages 17-62

    Wrzesinski, Krzysztof (et al.)

  • Protocol to Study the Role of Extracellular Vesicles During Induced Stem Cell Differentiation

    Pages 63-73

    Roballo, Kelly C. S. (et al.)

  • A Simplified Method for Three-Dimensional (3D) Porcine Preantral Follicles Culture Utilizing Hydrophobic Microbioreactors

    Pages 75-84

    Duda, Malgorzata (et al.)

  • Use of Transparent Liquid Marble: Microbioreactor to Culture Cardiospheres

    Pages 85-102

    Aalders, Jeffrey (et al.)

Buy this book

eBook 139,99 €
price for France (gross)
  • ISBN 978-1-0716-1246-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 179,34 €
price for France (gross)
  • ISBN 978-1-0716-1245-3
  • with online files
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
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Bibliographic Information

Bibliographic Information
Book Title
Next Generation Culture Platforms for Reliable In Vitro Models
Book Subtitle
Methods and Protocols
Editors
  • Tiziana A.L. Brevini
  • Alireza Fazeli
  • Kursad Turksen
Series Title
Methods in Molecular Biology
Series Volume
2273
Copyright
2021
Publisher
Springer US
Copyright Holder
The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-0716-1246-0
DOI
10.1007/978-1-0716-1246-0
Hardcover ISBN
978-1-0716-1245-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 307
Number of Illustrations
10 b/w illustrations, 59 illustrations in colour
Topics