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Human Cytomegaloviruses

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Andrew D. Yurochko

    1. Department of Microbiology and Immunology, Louisiana State University Health Sciences Center—Shreveport, Shreveport, USA

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2244)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Overview of Human Cytomegalovirus Pathogenesis

    • Heather L. Fulkerson, Maciej T. Nogalski, Donna Collins-McMillen, Andrew D. Yurochko
    Pages 1-18

  3. Distinct Properties of Human Cytomegalovirus Strains and the Appropriate Choice of Strains for Particular Studies

    • Giada Frascaroli, Christian Sinzger
    Pages 19-38

  4. Using Diploid Human Fibroblasts as a Model System to Culture, Grow, and Study Human Cytomegalovirus Infection

    • Elizabeth A. Fortunato
    Pages 39-50

  5. Using Primary Human Cells to Analyze Human Cytomegalovirus Biology

    • Emma Poole, Ian Groves, Sarah Jackson, Mark Wills, John Sinclair
    Pages 51-81

  6. Human Hematopoietic Long-Term Culture (hLTC) for Human Cytomegalovirus Latency and Reactivation

    • Megan Peppenelli, Jason Buehler, Felicia Goodrum
    Pages 83-101

  7. Collection and Isolation of CD14+ Primary Human Monocytes Via Dual Density Gradient Centrifugation as a Model System to Study Human Cytomegalovirus Infection and Pathogenesis

    • Bailey S. Mosher, Heather L. Fulkerson, Andrew D. Yurochko
    Pages 103-113

  8. Stable and Inducible Gene Knockdown in Primary Human Fibroblasts: A Versatile Tool to Study the Role of Human Cytomegalovirus Host Cell Factors

    • Anne-Charlotte Stilp, Patrick König, Myriam Scherer, Thomas Stamminger
    Pages 115-132

  9. Construction of Human Cytomegalovirus Mutants with Markerless BAC Mutagenesis

    • M. Zeeshan Chaudhry, Martin Messerle, Luka Čičin-Šain
    Pages 133-158

  10. Methods for Studying the Function of Cytomegalovirus GPCRs

    • Christine M. O’Connor, William E. Miller
    Pages 159-197

  11. Analysis of Cytomegalovirus Glycoprotein and Cellular Receptor Interactions

    • Jamil Mahmud, Gary C. Chan
    Pages 199-211

  12. Antibody-Independent Quantification of Cytomegalovirus Virion Protein Incorporation Using HiBiT

    • Iris K. A. Jones, Daniel N. Streblow
    Pages 213-232

  13. Using a Phosphoproteomic Screen to Profile Early Changes During HCMV Infection of Human Monocytes

    • Liudmila S. Chesnokova, Andrew D. Yurochko
    Pages 233-246

  14. A Generally Applicable CRISPR/Cas9 Screening Technique to Identify Host Genes Required for Virus Infection as Applied to Human Cytomegalovirus (HCMV) Infection of Epithelial Cells

    • Xiaofei E, Timothy F. Kowalik
    Pages 247-264

  15. Quantitative Electron Microscopy to Study HCMV Morphogenesis

    • Clarissa Read, Paul Walther, Jens von Einem
    Pages 265-289

  16. Detection of Cytomegalovirus Interleukin 10 (cmvIL-10) by Enzyme-Linked Immunosorbent Assay (ELISA)

    • Vivian P. Young, Margarette C. Mariano, Lionel Faure, Juliet V. Spencer
    Pages 291-299

  17. Techniques for Characterizing Cytomegalovirus-Encoded miRNAs

    • Nicole L. Diggins, Lindsey B. Crawford, Hillary M. Struthers, Lauren M. Hook, Igor Landais, Rebecca L. Skalsky et al.
    Pages 301-342

  18. Development of a huBLT Mouse Model to Study HCMV Latency, Reactivation, and Immune Response

    • Lindsey B. Crawford, Patrizia Caposio
    Pages 343-363

  19. Rodent Models of Congenital Cytomegalovirus Infection

    • Berislav Lisnić, Jelena Tomac, Djurdjica Cekinović, Stipan Jonjić, Vanda Juranić Lisnić
    Pages 365-401

  20. Recent Approaches and Strategies in the Generation of Anti-human Cytomegalovirus Vaccines

    • Suresh B. Boppana, William J. Britt
    Pages 403-463
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Keywords

About this book

This new edition explores and provides an update on the biology and pathogenesis of human cytomegalovirus infection.  Modern techniques that are currently being utilized to investigate the molecular aspects of viral infection, as well as how these new research studies are leading to new approaches to mitigate disease, are also provided. Given the key role the virus plays in significant acute and chronic human disease in all stages of life, from newborns to seniors, the need for clear methodologies to further explore the biology of HCMV infection and mitigation strategies is readily apparent. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Human Cytomegaloviruses: Methods and Protocols, Second Edition serves as an ideal scientific reference for basic and clinical scientists and medical personnel on the modern understanding of the pathobiology of the virus, and the approaches, techniques, and models to study human cytomegalovirus infection and disease.

Editors and Affiliations

  • Department of Microbiology and Immunology, Louisiana State University Health Sciences Center—Shreveport, Shreveport, USA

    Andrew D. Yurochko

Bibliographic Information

  • Book Title: Human Cytomegaloviruses

  • Book Subtitle: Methods and Protocols

  • Editors: Andrew D. Yurochko

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1111-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1110-4Published: 09 February 2021

  • Softcover ISBN: 978-1-0716-1113-5Published: 09 February 2022

  • eBook ISBN: 978-1-0716-1111-1Published: 08 February 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 468

  • Number of Illustrations: 15 b/w illustrations, 26 illustrations in colour

  • Topics: Virology, Human Physiology, Biomedicine, general

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