Toxicity Assessment

1. Front Matter

   Pages i-xii

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2. The Single-Cell Gel Electrophoresis Genotoxin Sensitivity Assay

   • Franziska Ferk, Miroslav Mišík, Halh Al-Serori, Armen Nersesyan, Georg Wultsch, Siegfried Knasmueller

   Pages 1-12

3. Skin Sensitization Tests: The LLNA and the RhE IL-18 Potency Assay

   • Emanuela Corsini, Sue Gibbs, Erwin Roggen, Ian Kimber, David A. Basketter

   Pages 13-29

4. Skin Equivalent Models: Protocols for In Vitro Reconstruction for Dermal Toxicity Evaluation

   • Tatiana do Nascimento Pedrosa, Carolina Motter Catarino, Paula Comune Pennacchi, Silvia Berlanga de Moraes Barros, Silvya Stuchi Maria-Engler

   Pages 31-41

5. Intravital Microscopy: A Tool to Investigate the Toxicity in the Immune System , Vessel Rheology , and Xenobiotic Distribution

   • Cristina Bichels Hebeda, Éric Diego Barioni, Sandra Helena Poliselli Farsky

   Pages 43-55

6. Cell-Based Biosensor to Visualize Nitric Oxide Release from Living Cells for Toxicity Assessment

   • Moritoshi Sato, Yoshio Umezawa

   Pages 57-64

7. In Vitro Contraction of Isolated Cauda Epididymal Duct Smooth Muscle as a Complimentary Approach to Physiological, Pathological, Toxicological, and Pharmacological Studies on Epididymal Function

   • Cibele S. Borges, Luiz Ricardo A. Kiguti, André S. Pupo, Wilma G. Kempinas

   Pages 65-76

8. Early Life Stage Assays in Zebrafish

   • Flávia Renata Abe, Klaus Alvaro Guerrieri Accoroni, Carlos Gravato, Danielle Palma de Oliveira

   Pages 77-92

9. Assessment of Urothelial Cytotoxicity at Morphological and Molecular Levels: Urinary Bladder as Target Organ

   • Thania Rios Rossi Lima, Bianca Camargo Penteado Sales, Shadia Muhammad Ihlaseh Catalano

   Pages 93-102

10. Evaluation of Development of the Rat Uterus as a Toxicity Biomarker

    • Marlise Guerrero Schimpf, María M. Milesi, Enrique H. Luque, Jorgelina Varayoud

    Pages 103-117

11. Acetylcholinesterase (AChE) Activity in Embryos of Zebrafish

    • Carlos Gravato, Flávia Renata Abe, Danielle Palma de Oliveira, Amadeu M. V. M. Soares, Inês Domingues

    Pages 119-124

12. Ecotoxicity Assays Using Freshwater Planarians

    • João Luís Teixeira Pestana, Pearl Ugochi Ofoegbu

    Pages 125-137

13. Toxicogenomic Analysis

    • Taisa Magnani Dinamarco, Paula Fagundes de Gouvêa, Emerson de Souza Santos, Lucas Oliveira Sousa

    Pages 139-174

14. Strategy Combining Nonanimal Methods for Ocular Toxicity Evaluation

    • Marize Campos Valadares, Gisele Augusto Rodrigues de Oliveira, Renato Ivan de Ávila, Artur Christian Garcia da Silva

    Pages 175-195

15. Immunocytochemistry Analysis of HepG2 Cell 3D Culture Encapsulated as Spheroids in Alginate Beads

    • Raul Ghiraldelli Miranda, Elisa Raquel Anastácio Ferraz, Lilian Cristina Pereira, Daniel Junqueira Dorta

    Pages 197-206

16. Oxygen Consumption Evaluation: An Important Indicator of Metabolic State, Cellular Function , and Cell Fate Along Neural Deregulation

    • Mariana Dutra Brito, Luiz Felipe Souza e Silva, Amanda Siena, Miruna Chipara, Sovan Sarkar, Tatiana Rosado Rosenstock

    Pages 207-230

17. Mitochondrial Bioenergetic Assays as a Standard Protocol for Toxicological and Metabolic Assessment

    • João Soeiro Teodoro, Anabela Pinto Rolo, Carlos Manuel Marques Palmeira

    Pages 231-241

18. In Vitro Apoptotic Cell Death Assessment

    • Lilian Cristina Pereira, Alecsandra Oliveira de Souza, Raul Ghiraldelli Miranda, Daniel Junqueira Dorta

    Pages 243-261

19. Back Matter

    Pages 263-266

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This book collects protocols from different areas of knowledge to assist in the identification of toxic effects exerted by different xenobiotics. At the same time as classical techniques are presented, modern techniques with alternative models to the use of animals are also presented. Given the ever-increasing exposure to different compounds and their effects on population health, the assessment of multiple endpoints is of utmost importance for better risk assessment, and this collection addresses that need. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Toxicity Assessment: Methods and Protocols aims to serve researchers in this vast field of science as they seek to better understand the mechanisms of action of different xenobiotics.

• Toxicological tests
• Xenobiotics
• Chemical substances
• Toxic effects
• Alternative models
• Risk assessment

• MitoLab, Department of Life Sciences, Center for Neurosciences and Cell Biology of the University of Coimbra, Coimbra, Portugal

  Carlos Manuel Marques Palmeira

• School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil

  Danielle Palma de Oliveira

• Department of Chemistry, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil

  Daniel Junqueira Dorta

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