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Listeria Monocytogenes

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Edward M. Fox

    1. Department of Applied Sciences, Northumbria University, Newcastle-upon-Tyne, UK

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  2. Hélène Bierne

    1. INRAE, AgroParisTech, Micalis Institute,, Université Paris-Saclay, Jouy-en-Josas, France

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  3. Beatrix Stessl

    1. Institute of Food Safety Food Technology and Veterinary Public Health, University of Veterinary Medicine Vienna, Vienna, Austria

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2220)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
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  2. Detection, Quantification, and Confirmation

    1. Front Matter

      Pages 1-1
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    2. Traditional Methods of Analysis for Listeria monocytogenes

      • Rui Magalhães, Cristina Mena, Vânia Ferreira, Gonçalo Almeida, Joana Silva, Paula Teixeira
      Pages 3-16

    3. MALDI-ToF MS: A Rapid Methodology for Identifying and Subtyping Listeria monocytogenes

      • Snehal R. Jadhav, Rohan M. Shah, Enzo A. Palombo
      Pages 17-29

    4. Sample Preparation for qPCR Detection of Listeria from Food

      • Patrick Mester, Anna Kristina Witte, Peter Rossmanith
      Pages 31-40

    5. qPCR Validation on the Basis of the Listeria monocytogenes prfA Assay

      • Anna Kristina Witte, Patrick Mester, Peter Rossmanith
      Pages 41-53

  4. Genotypic and Phenotypic Characterization

    1. Front Matter

      Pages 105-105
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    2. High-Throughput Characterization of Listeria monocytogenes Using the OmniLog Phenotypic Microarray

      • Laura Luque-Sastre, Kieran Jordan, Séamus Fanning, Edward M. Fox
      Pages 107-113

    3. High-Throughput Screening of Biofilm Formation of Listeria monocytogenes on Stainless Steel Coupons Using a 96-Well Plate Format

      • Jessica A. Gray, P. Scott Chandry, John P. Bowman, Edward M. Fox
      Pages 115-122

    4. Confocal Laser Microscopy Analysis of Listeria monocytogenes Biofilms and Spatially Organized Communities

      • Maud Darsonval, Marina Grégoire, Julien Deschamps, Romain Briandet
      Pages 123-136

    5. Extraction and Preparation of Listeria monocytogenes Subproteomes for Mass Spectrometry Analysis

      • Tiago Santos, Michel Hébraud
      Pages 137-153

  5. Strain Manipulation

    1. Front Matter

      Pages 155-155
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    2. Extraction and Analysis of Plasmid DNA from Listeria monocytogenes

      • Aidan Casey, Olivia McAuliffe
      Pages 157-163

    3. Generation of Nonpolar Deletion Mutants in Listeria monocytogenes Using the “SOEing” Method

      • Kathrin Rychli, Eva Wagner, Caitriona M. Guinane, Karen Daly, Colin Hill, Paul D. Cotter
      Pages 165-175

    4. Mutant Construction and Integration Vector-Mediated Genetic Complementation in Listeria monocytogenes

      • Cameron Parsons, Reha Azizoglu, Driss Elhanafi, Sophia Kathariou
      Pages 177-185

  6. Host-Pathogen Interactions

    1. Front Matter

      Pages 187-187
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Keywords

About this book

This updated book explores a wide repertoire of tools and approaches that have been created, modified, and applied to the study of L. monocytogenes, forming the basis of our understanding of the bacterium today. Many of these key experimental techniques are gathered together herein. The volume presents aspects such as clinical disease and host-pathogen interactions, as well as the study of biofilms which present a significant challenge for control of the organism in the food processing environment. The topics covered in this edition also include sampling in order to isolate Listeria, methods for their identification and characterization, methods for gene manipulation, and methods for control of the organism. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshootingand avoiding known pitfalls. 

Authoritative and up-to-date, Listeria monocytogenes: Methods and Protocols, Second Edition aims to contribute toward the harmonization of methods used to study this important bacterium, and to be of particular interest to Listeria research both in relation to food association and control as well as clinical microbiology.

Editors and Affiliations

  • Department of Applied Sciences, Northumbria University, Newcastle-upon-Tyne, UK

    Edward M. Fox

  • INRAE, AgroParisTech, Micalis Institute,, Université Paris-Saclay, Jouy-en-Josas, France

    Hélène Bierne

  • Institute of Food Safety Food Technology and Veterinary Public Health, University of Veterinary Medicine Vienna, Vienna, Austria

    Beatrix Stessl

Bibliographic Information

  • Book Title: Listeria Monocytogenes

  • Book Subtitle: Methods and Protocols

  • Editors: Edward M. Fox, Hélène Bierne, Beatrix Stessl

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0982-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0981-1Published: 26 September 2020

  • Softcover ISBN: 978-1-0716-0984-2Published: 27 September 2021

  • eBook ISBN: 978-1-0716-0982-8Published: 25 September 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 260

  • Number of Illustrations: 24 b/w illustrations, 30 illustrations in colour

  • Topics: Bacteriology, Food Science, Food Microbiology

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