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Bio-Carrier Vectors

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Kumaran Narayanan

    1. School of Science, Monash University Malaysia, Bandar Sunway, Malaysia

    View editor publications

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2211)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
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  2. Bacterial Vectors

    1. Front Matter

      Pages 1-1
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    2. Improving E. coli Bactofection by Expression of Bacteriophage ΦX174 Gene E

      • Dongwon Park, Andrew Hill, Blaine A. Pfeifer
      Pages 3-14

    3. Improved DNA Delivery Efficiency of Bacterial Vectors by Co-Delivery with Exogenous Lipid and Antimicrobial Reagents

      • Andrew N. Osahor, Kumaran Narayanan
      Pages 15-27

    4. Visualization of Bacteria-Mediated Gene Delivery Using High-Resolution Electron and Confocal Microscopy

      • Andrew N. Osahor, Allan Wee Ren Ng, Kumaran Narayanan
      Pages 29-40

  3. Nanoparticles

    1. Front Matter

      Pages 41-41
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    2. Assessing Nucleic Acid: Cationic Nanoparticle Interaction for Gene Delivery

      • Moganavelli Singh
      Pages 43-55

    3. Catanionic Hybrid Lipid Nanovesicles for Improved Bioavailability and Efficacy of Chemotherapeutic Drugs

      • Xuemei Xie, Dan He, Yan Wu, Tingting Wang, Cailing Zhong, Jingqing Zhang
      Pages 57-68

    4. Magnetic Nanoparticles as a Carrier of dsRNA for Gene Therapy

      • MaÅ‚gorzata Grabowska, Bartosz F. GrzeÅ›kowiak, Katarzyna Rolle, RadosÅ‚aw MrówczyÅ„ski
      Pages 69-81

  4. Peptide Vehicles

    1. Front Matter

      Pages 83-83
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    2. Generation of Functional Insulin-Producing Cells from Mouse Embryonic Stem Cells Through Protein Transduction of Transcription Factors

      • Taku Kaitsuka, Kazuhito Tomizawa
      Pages 85-96

    3. Cardiac Targeting Peptide : From Identification to Validation to Mechanism of Transduction

      • Kyle S. Feldman, Maria P. Pavlou, Maliha Zahid
      Pages 97-112

    4. Lactoferricin-Derived L5a Cell-Penetrating Peptide for Delivery of DNA into Cells

      • Natalie J. Holl, Moumita Dey, Yue-Wern Huang, Shiow-Her Chiou, Han-Jung Lee
      Pages 113-121

    5. Delivery of Peptide Nucleic Acids Using an Argininocalix[4]arene as Vector

      • Alessia Finotti, Jessica Gasparello, Alessandro Casnati, Roberto Corradini, Roberto Gambari, Francesco Sansone
      Pages 123-143

  5. Hybrid Carriers and Other Bio Agents

    1. Front Matter

      Pages 145-145
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    2. Cell-Derived Nanovesicles as Exosome-Mimetics for Drug Delivery Purposes: Uses and Recommendations

      • Yi-Hsuan Ou, Shui Zou, Wei Jiang Goh, Jiong-Wei Wang, Matthias Wacker, Bertrand Czarny et al.
      Pages 147-170

    3. Hydrogel Beads of Natural Polymers as a Potential Vehicle for Colon-Targeted Drug Delivery

      • Janarthanan Pushpamalar, Thenapakiam Sathasivam, Michelle Claire Gugler
      Pages 171-182

    4. Scaffold-Based Delivery of CRISPR/Cas9 Ribonucleoproteins for Genome Editing

      • Wai Hon Chooi, Jiah Shin Chin, Sing Yian Chew
      Pages 183-191

    5. Correction of Hemoglobin E /Beta-Thalassemia Patient-Derived iPSCs Using CRISPR/Cas9

      • Methichit Wattanapanitch
      Pages 193-211

    6. CRISPR/Cas9-Mediated GFP Reporter Knock-in in K562 and Raji Cell Lines for Tracking Immune Cell Killing Assay

      • Nontaphat Thongsin, Methichit Wattanapanitch
      Pages 213-229
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Keywords

About this book

This detailed book explores techniques used in research efforts to adopt and combine the best capabilities from natural and artificial vector systems to assemble improved delivery technologies aimed for specific applications. The collection discusses vectors other than the traditional viral and non-viral systems and explores ideas for blending the best features of bacteria, nanoparticles, peptides, and hybrid systems for the delivery of biomaterials into cells, as well as a chapter on the application of shotgun proteomics and mass spectrometry as a tool to analyze the proteomic profile changes in cells that result from these interventions. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical,Bio-Carrier Vectors: Methods and Protocols serves as an ideal guide for researchers working toward harnessing the power of multiple vectors in gene and drug delivery.

Editors and Affiliations

  • School of Science, Monash University Malaysia, Bandar Sunway, Malaysia

    Kumaran Narayanan

Bibliographic Information

  • Book Title: Bio-Carrier Vectors

  • Book Subtitle: Methods and Protocols

  • Editors: Kumaran Narayanan

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0943-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0942-2Published: 18 December 2020

  • Softcover ISBN: 978-1-0716-0945-3Published: 19 December 2021

  • eBook ISBN: 978-1-0716-0943-9Published: 17 December 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 243

  • Number of Illustrations: 10 b/w illustrations, 32 illustrations in colour

  • Topics: Chemistry/Food Science, general, Biotechnology, Biomaterials, Biological Techniques

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