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Coronaviruses

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Helena J. Maier

    1. The Pirbright Institute, Surrey, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. Erica Bickerton

    1. The Pirbright Institute, Surrey, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2203)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Coronaviruses: An Updated Overview of Their Replication and Pathogenesis

    • Yuhang Wang, Matthew Grunewald, Stanley Perlman
    Pages 1-29

  3. Coronavirus Detection, Discovery, and Evolution

    1. Front Matter

      Pages 31-31
      Download chapter PDF

    2. Discovery of Novel Coronaviruses in Rodents

      • Theocharis Tsoleridis, Jonathan K. Ball
      Pages 33-40

    3. Detection and Discovery of Coronaviruses in Wild Bird Populations

      • Chantal J. Snoeck, Siamak Zohari
      Pages 41-53

    4. Competitive ELISA for the Detection of Serum Antibodies Specific for Middle East Respiratory Syndrome Coronavirus (MERS-CoV)

      • Shuetsu Fukushi
      Pages 55-65

    5. Whole-Genome Sequencing Protocols for IBV and Other Coronaviruses Using High-Throughput Sequencing

      • Graham L. Freimanis, Michael S. Oade
      Pages 67-74

  4. Propagation and Titration of Coronaviruses

    1. Front Matter

      Pages 75-75
      Download chapter PDF

    2. Isolation and Tissue Culture Adaptation of Porcine Deltacoronavirus: A Case Study

      • Hui Hu, Kwonil Jung, Scott P. Kenney, Linda J. Saif
      Pages 77-88

    3. The Preparation of Chicken Kidney Cell Cultures for Virus Propagation

      • Elena Lokhman, Srijana Rai, William Matthews
      Pages 89-95

    4. The Preparation of Chicken Tracheal Organ Cultures and Their Application for Ciliostasis Test, Growth Kinetics Studies, and Virus Propagation

      • Giulia Dowgier, Erica Bickerton
      Pages 97-106

    5. Isolation and Propagation of Coronaviruses in Embryonated Eggs

      • James S. Guy
      Pages 107-117

    6. Well-Differentiated Primary Mammalian Airway Epithelial Cell Cultures

      • Mitra Gultom, Laura Laloli, Ronald Dijkman
      Pages 119-134

    7. Quantification of Coronaviruses by Titration In Vitro and Ex Vivo

      • Giulia Dowgier, Helena J. Maier
      Pages 135-143

  5. Manipulating the Genomes of Coronaviruses

    1. Front Matter

      Pages 145-145
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    2. Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitis Coronaviruses

      • Sarah Keep, Paul Britton, Erica Bickerton
      Pages 147-165

    3. In-Yeast Assembly of Coronavirus Infectious cDNA Clones Using a Synthetic Genomics Pipeline

      • Tran Thi Nhu Thao, Fabien Labroussaa, Nadine Ebert, Joerg Jores, Volker Thiel
      Pages 167-184

  6. Studying Virus-Host Interactions

    1. Front Matter

      Pages 185-185
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    2. Proximity Labeling for the Identification of Coronavirus–Host Protein Interactions

      • Philip V’kovski, Silvio Steiner, Volker Thiel
      Pages 187-204

    3. Using Yeast to Identify Coronavirus–Host Protein Interactions

      • Stuart Weston, Matthew Frieman
      Pages 205-221
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Keywords

About this book

This detailed new edition provides a comprehensive collection of protocols applicable to all members of the Coronavirinae sub-family currently and that are also transferrable to other fields of virology. Beginning with a section on detection, discovery, and evolution, the volume continues with coverage of propagation and titration of coronaviruses, genome manipulation, study of virus-host interactions, as well as imaging coronavirus infections. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Coronaviruses: Methods and Protocols, Second Edition serves as a valuable guide to researchers working to identify and control viruses with increased potential to cross the species barrier and to develop the diagnostics, vaccines, and antiviral therapeutics that are required to manage future outbreaks in both humans and animals.

Editors and Affiliations

  • The Pirbright Institute, Surrey, UK

    Helena J. Maier, Erica Bickerton

Bibliographic Information

  • Book Title: Coronaviruses

  • Book Subtitle: Methods and Protocols

  • Editors: Helena J. Maier, Erica Bickerton

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0900-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0899-9Published: 25 August 2020

  • Softcover ISBN: 978-1-0716-0902-6Published: 25 August 2021

  • eBook ISBN: 978-1-0716-0900-2Published: 24 August 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 278

  • Number of Illustrations: 16 b/w illustrations, 25 illustrations in colour

  • Topics: Virology

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