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DNA Vaccines

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Ângela Sousa

    1. CICS-UBI – Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2197)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
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  2. Vector Design for Vaccination

    1. Front Matter

      Pages 1-1
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    2. A Novel Cre Recombinase-Mediated In Vivo Minicircle (CRIM) DNA Vaccine Platform for Veterinary Application

      • Yanlong Jiang, Guilian Yang, Chunfeng Wang
      Pages 3-12

    3. Vaccination with Messenger RNA: A Promising Alternative to DNA Vaccination

      • István Tombácz, Drew Weissman, Norbert Pardi
      Pages 13-31

    4. Impact of a Plasmid DNA-Based Alphavirus Vaccine on Immunization Efficiency

      • Kenneth Lundstrom
      Pages 33-47

  3. DNA Vaccine Adjuvants and Immunostimulatory Response

    1. Front Matter

      Pages 49-49
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    2. CpG Oligonucleotides as Vaccine Adjuvants

      • Neslihan Kayraklioglu, Begum Horuluoglu, Dennis M. Klinman
      Pages 51-85

    3. Molecular Adjuvants for DNA Vaccines : Application, Design, Preparation, and Formulation

      • Ailar Sabbaghi, Amir Ghaemi
      Pages 87-112

    4. Assessing Antigen-Specific Cellular Immune Responses upon HIV /SIV Plasmid DNA Vaccination in the Nonhuman Primate Model

      • Xintao Hu, Barbara K. Felber, Antonio Valentin
      Pages 113-131

  4. Biotechnological Processes to Obtain DNA Vaccines

    1. Front Matter

      Pages 133-133
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    2. Enhanced Biosynthesis of Plasmid DNA from Escherichia coli Applying Experimental Design

      • Luís A. Passarinha
      Pages 135-150

    3. Primary Purification of Plasmid DNA Using Differential Isopropanol Precipitation

      • Alexandra Wagner, A. Rita Silva-Santos, Sara Sousa Rosa, Sophie Gierak, Ana M. Azevedo, Duarte Miguel F. Prazeres
      Pages 151-165

    4. Scale-Up of Plasmid DNA Downstream Process Based on Chromatographic Monoliths

      • Urh Černigoj, Aleš Štrancar
      Pages 167-192

    5. Purification of Plasmid DNA by Multimodal Chromatography

      • A. Rita Silva-Santos, Sara Sousa Rosa, Duarte Miguel F. Prazeres, Ana M. Azevedo
      Pages 193-205

    6. Minicircle DNA Vaccine Purification and E7 Antigen Expression Assessment

      • Ana M. Almeida, Dalinda Eusébio, João A. Queiroz, Fani Sousa, Ângela Sousa
      Pages 207-222

  5. DNA Vaccine Delivery

    1. Front Matter

      Pages 223-223
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    2. Tumor-Specific CD8+ T-Cell Responses Induced by DNA Vaccination

      • Pablo Cáceres-Morgado, Alvaro Lladser
      Pages 225-239

    3. Therapeutic DNA Vaccine Against HPV16-Associated Cancer

      • Meihua Yu, Janin Chandra
      Pages 241-252

    4. Bulk and Microfluidic Synthesis of Stealth and Cationic Liposomes for Gene Delivery Applications

      • Lucimara Gaziola de la Torre, Amanda Costa Silva Noronha Pessoa, Bruna Gregatti de Carvalho, Thiago Bezerra Taketa, Ismail Eş, Gabriel Perli
      Pages 253-269

    5. Conception of Plasmid DNA and Polyethylenimine Delivery Systems with Potential Application in DNA Vaccines Field

      • Diana Costa, Ângela Sousa, Rúben Faria, Ana Raquel Neves, João A. Queiroz
      Pages 271-284
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Keywords

About this book

This volume details practical procedures on the latest DNA vaccine technology. Chapters guide readers through methods and protocols on DNA vaccine design, the adjuvant influence, production and purification methodologies, delivery systems, and approaches of the influence of DNA vaccines in the immunological response performance and in the cancer immunotherapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, DNA Vaccines: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • CICS-UBI – Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal

    Ângela Sousa

Bibliographic Information

  • Book Title: DNA Vaccines

  • Book Subtitle: Methods and Protocols

  • Editors: Ângela Sousa

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0872-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0871-5Published: 22 August 2020

  • Softcover ISBN: 978-1-0716-0874-6Published: 22 August 2021

  • eBook ISBN: 978-1-0716-0872-2Published: 21 August 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 335

  • Number of Illustrations: 14 b/w illustrations, 25 illustrations in colour

  • Topics: Immunology, Human Genetics

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