Overview
- Editors:
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Hailing Jin
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Department of Plant Pathology and Microbiology, Center for Plant Cell Biology and Institute for Integrative Genome Biology, University of California, Riverside, USA
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Isgouhi Kaloshian
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Department of Nematology, University of California, Riverside, USA
- Includes cutting-edge techniques
- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
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Table of contents (16 protocols)
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- Veerendra Sharma, Wenguang Zheng, Jun Huang, David E. Cook
Pages 1-18
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- Xueqing Maggie Lu, Karine Le Roch
Pages 19-33
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- Marta Barcala, Carmen Fenoll, Carolina Escobar
Pages 35-43
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- Lena Krüßel, Steffen Ostendorp, Anna Ostendorp, Julia Kehr
Pages 45-51
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- Jakub Dolata, Andrzej Zielezinski, Agata Stepien, Katarzyna Kruszka, Dawid Bielewicz, Andrzej Pacak et al.
Pages 53-77
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- Dennis Chang, Lorrayne Serra, Dihong Lu, Ali Mortazavi, Adler Dillman
Pages 79-99
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- Erin L. Sternburg, Fedor V. Karginov
Pages 101-115
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- Luis A. Jimenez, Wenwan Zhong
Pages 117-124
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- Weixiong Zhang, Xuefeng Zhou, Xiang Zhou, Jing Xia
Pages 125-131
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- Xiaozhen Yao, Hai Huang, Lin Xu
Pages 143-154
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- Ankur R. Bhardwaj, Ritu Pandey, Manu Agarwal, Surekha Katiyar-Agarwal
Pages 155-183
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- Mélanie Ribeiro Lopes, Pierre Simonet, Gabrielle Duport, Karen Gaget, Séverine Balmand, Akiko Sugio et al.
Pages 185-198
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- Shou-Qiang Ouyang, Gyungsoon Park, Hui-Min Ji, Katherine A. Borkovich
Pages 199-212
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- Sonali Chaturvedi, A. L. N. Rao
Pages 213-218
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Back Matter
Pages 231-233
About this book
This updated book covers a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches. It includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, organelle RNAs, endosymbiont RNAs, and alternatively spliced RNA variants from various organisms. RNA editing and the computational data processing for genome-wide datasets is also discussed. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, RNA Abundance Analysis: Methods and Protocols, Second Edition provides a comprehensive set of techniques and methods on isolating and analyzing mRNAs, small RNAs, and other RNA variants, in order to assist researchers in their gene expression studies.
Editors and Affiliations
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Department of Plant Pathology and Microbiology, Center for Plant Cell Biology and Institute for Integrative Genome Biology, University of California, Riverside, USA
Hailing Jin
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Department of Nematology, University of California, Riverside, USA
Isgouhi Kaloshian
