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Schistosoma mansoni

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. David J. Timson

    1. School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton, UK

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2151)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Praziquantel: An Enigmatic, Yet Effective, Drug

    • David J. Timson
    Pages 1-8

  3. Computer-Aided Drug Design for the Identification of Novel Antischistosomal Compounds

    • Jéssika de Oliveira Viana, Marcus T. Scotti, Luciana Scotti
    Pages 9-26

  4. Immunofluorescent Localization of Proteins in Schistosoma mansoni

    • Rashika El Ridi, Hatem Tallima
    Pages 27-41

  5. Sensitive Fluorescence In Situ Hybridization on Semithin Sections of Adult Schistosoma mansoni Using DIG-Labeled RNA Probes

    • Lenka Ulrychová, Martin Horn, Jan Dvořák
    Pages 43-53

  6. Collection of Excretory/Secretory Products from Individual Developmental Stages of the Blood Fluke Schistosoma mansoni

    • Adrian Leontovyč, Lenka Ulrychová, Martin Horn, Jan Dvořák
    Pages 55-63

  7. Labeling of the Schistosoma mansoni Tegument

    • George Wendt, James J. Collins
    Pages 65-74

  8. A Procedure for Analyzing the Proteomic Proteomics Profile of Schistosoma mansoni Cercariae

    • Dilhan Perera, Makan Golizeh, Momar Ndao
    Pages 75-84

  9. Proteomic Analysis of Schistosoma mansoni Tegumental Proteins

    • Mark S. Pearson, Alex Loukas, Javier Sotillo
    Pages 85-92

  10. Measuring Histone Modifications in the Human Parasite Schistosoma mansoni

    • Ronaldo de Carvalho Augusto, David Roquis, Marion AL Picard, Cristian Chaparro, Celine Cosseau, Christoph Grunau
    Pages 93-107

  11. Step-by-Step Bioinformatics Analysis of Schistosoma mansoni Long Non-coding RNA Sequences

    • Lucas F. Maciel, Sergio Verjovski-Almeida
    Pages 109-133

  12. Identification of Transposable Elements in Schistosoma mansoni

    • Gisele S. Philippsen, Ricardo DeMarco
    Pages 135-144

  13. Structural and Functional Characterization of Schistosoma mansoni Cathepsin B1

    • Adéla Jílková, Martin Horn, Michael Mareš
    Pages 145-158

  14. Characterization of Schistosoma mansoni Dihydrofolate Reductase (DHFR)

    • Vitor Hugo B. Serrão, Jéssica F. Scortecci, Humberto D’Muniz Pereira
    Pages 159-172

  15. Characterization of Schistosoma mansoni Glutamate-Gated Chloride Channels

    • Timothy Lynagh
    Pages 173-183

  16. A Modified ELISA Method to Evaluate the Interaction of Schistosoma mansoni Proteins with Plasminogen

    • Luis G. V. Fernandes, Rafaela S. Fernandes, Ana L. T. O. Nascimento, Luciana C. C. Leite
    Pages 185-195

  17. Biophysical Analysis of Schistosoma mansoni Septins

    • Ana Eliza Zeraik, Marina Gabriel Fontes, Ricardo DeMarco
    Pages 197-210

  18. Crystallographic Studies of Triosephosphate Isomerase from Schistosoma mansoni

    • Pedro Jimenez-Sandoval, Eduardo Castro-Torres, Corina Diaz-Quezada, Luis G. Brieba
    Pages 211-218

  19. Luminescence-Based, Low- and Medium-Throughput Assays for Drug Screening in Schistosoma mansoni Larval Stage

    • Alessandra Guidi, Roberto Gimmelli, Alberto Bresciani, Giovina Ruberti
    Pages 219-227

  20. Testing of Schistosoma mansoni Vaccine Targets

    • Weidong Zhang, Adebayo Molehin, Parth Patel, Eunjee Kim, Adam Peña, Afzal A. Siddiqui
    Pages 229-262
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Keywords

About this book

This volume details protocols for studying Schistosoma mansoni.  Chapters guide readers through reviews on current drugs and drug discovery, methods to interrogate the cell biology of the worm, protocols to look at proteome level changes, prepare and work with specific proteins from S. mansoni, describes a drug screening, and how to test potential vaccine targets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Schistosoma mansoni: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton, UK

    David J. Timson

Bibliographic Information

  • Book Title: Schistosoma mansoni

  • Book Subtitle: Methods and Protocols

  • Editors: David J. Timson

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0635-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0634-6Published: 26 May 2020

  • Softcover ISBN: 978-1-0716-0637-7Published: 26 May 2021

  • eBook ISBN: 978-1-0716-0635-3Published: 25 May 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 265

  • Number of Illustrations: 23 b/w illustrations, 36 illustrations in colour

  • Topics: Parasitology, Pharmacology/Toxicology, Chemistry/Food Science, general, Biotechnology, Biomaterials

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