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Axon Degeneration

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Elisabetta Babetto

    1. Department of Biochemistry, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, USA

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2143)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xi
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  2. Introduction

    1. Front Matter

      Pages 1-1
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    2. Axon Degeneration: Which Method to Choose?

      • Michael P. Coleman
      Pages 3-12

  3. In Vitro Assays

    1. Front Matter

      Pages 13-13
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    2. Axon Degeneration Assays in Superior Cervical Ganglion Explant Cultures

      • Andrea Loreto, Jonathan Gilley
      Pages 15-24Open Access

    3. Microinjection of Superior Cervical Ganglion Neurons for Studying Axon Degeneration

      • Jonathan Gilley, Andrea Loreto
      Pages 25-39Open Access

    4. Assessing Axonal Degeneration in Embryonic Dorsal Root Ganglion Neurons In Vitro

      • Jung Eun Shin, Yongcheol Cho
      Pages 41-54

    5. Viral Transduction of DRG Neurons

      • Yo Sasaki
      Pages 55-62

    6. Planning and Analysis of Axon Degeneration Screening Experiments

      • Lyndah Lovell, John Bramley, William Buchser
      Pages 63-82

    7. A Microfluidic Culture Platform to Assess Axon Degeneration

      • Yu Yong, Christopher Hughes, Christopher Deppmann
      Pages 83-96

    8. A Schwann Cell–Neuron Coculture System to Study Neuron–Glia Interaction During Axon Degeneration

      • Elisabetta Babetto
      Pages 97-110

    9. Establishing Myelinating Cocultures Using Human iPSC-Derived Sensory Neurons to Investigate Axonal Degeneration and Demyelination

      • Alex J. Clark
      Pages 111-129

  4. Ex Vivo Assays

    1. Front Matter

      Pages 131-131
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    2. Preparation of Organotypic Hippocampal Slice Cultures for the Study of CNS Disease and Damage

      • Claire S. Durrant
      Pages 133-144

    3. Organotypic Culture Assay for Neuromuscular Synaptic Degeneration and Function

      • Kosala N. Dissanayake, Robert Chang-Chih Chou, Rosalind Brown, Richard R. Ribchester
      Pages 145-157

    4. Intracellular Recordings of Postsynaptic Voltage Responses at the Drosophila Neuromuscular Junction

      • Vera Valakh, Johanna G. Flyer-Adams
      Pages 159-168

    5. Ex Vivo Studies of Optic Nerve Axon Electrophysiology

      • Chinthasagar Bastian, Sylvain Brunet, Selva Baltan
      Pages 169-177

    6. Ex Vivo Analysis of Axonal Degeneration Using Sciatic and Optic Nerve Preparations

      • Rodrigo López-Leal, Felipe A. Court
      Pages 179-189

    7. Measuring Bioenergetic Signatures of Peripheral Nerve Segments by Extracellular Flux Analysis

      • Bogdan Beirowski
      Pages 191-203

  5. In Vivo Assays

    1. Front Matter

      Pages 205-205
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Keywords

About this book

This book is a collection of classical as well as innovative methods used to investigate axon degeneration with a particular focus on addressing the common challenges encountered while performing these procedures. Particular attention is devoted to the study of axon loss in several model organisms, as each poses unique challenges and provides powerful advantages. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Axon Degeneration: Methods and Protocols is an ideal guide for facilitating the application and further development of these protocols, which will help the scientific community tackle important questions regarding axon degeneration.


Chapters 2, 3, and 20 are availableOpen Access under a Creative Commons Attribution 4.0 International License via link.springer.com.


Editors and Affiliations

  • Department of Biochemistry, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, USA

    Elisabetta Babetto

Bibliographic Information

  • Book Title: Axon Degeneration

  • Book Subtitle: Methods and Protocols

  • Editors: Elisabetta Babetto

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0585-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0584-4Published: 11 June 2020

  • Softcover ISBN: 978-1-0716-0587-5Published: 11 June 2020

  • eBook ISBN: 978-1-0716-0585-1Published: 10 June 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 340

  • Number of Illustrations: 22 b/w illustrations, 58 illustrations in colour

  • Topics: Neurobiology, Neurosciences

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