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Methods in Molecular Biology
cover

Fasciola hepatica

Methods and Protocols

Editors: Cancela, Martin, Maggioli, Gabriela (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook 119,99 €
price for France (gross)
  • ISBN 978-1-0716-0475-5
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Hardcover 158,24 €
price for France (gross)
  • ISBN 978-1-0716-0474-8
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About this book

This volume described basic and advanced protocols to study F. hepatica parasite biology. Chapters guide readers through protocols on different developmental stages of F. hepatica, characterize tegumental, secreted proteins, spatial and temporal gene expression, immunological effects of ESP on macrophages, eosinophil apoptosis, macrophages alternative activation, toll-like receptor interactions, obtain peritoneal and splenic dendritic cells of mice, and protocols to detect F. hepatica albendazole resistance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Fasciola hepatica: Methods and Protocols provides different topics in areas such a biochemistry, immunology, molecular biology, microscopy, vaccinology in order to extend the interest in this book to research community working with Fasciola hepatica and related trematodes.

Table of contents (17 chapters)

Table of contents (17 chapters)
  • Maintenance of Life Cycle Stages of Fasciola hepatica in the Laboratory

    Pages 1-14

    Gayo, Valeria (et al.)

  • Microscopical Techniques to Analyze the Hepatic and Peritoneal Changes Caused by Fasciola hepatica Infection

    Pages 15-25

    Ruiz-Campillo, M. T. (et al.)

  • Isolation of Secreted and Tegumental Surface Proteins from Fasciola hepatica

    Pages 27-36

    Torre-Escudero, Eduardo (et al.)

  • Isolation and Analysis of Fasciola hepatica Extracellular Vesicles

    Pages 37-50

    Galiano, Alicia (et al.)

  • Cloning and Heterologous Expression of Protein-Coding Sequences in Escherichia coli

    Pages 51-66

    Cancela, Martin (et al.)

Buy this book

eBook 119,99 €
price for France (gross)
  • ISBN 978-1-0716-0475-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 158,24 €
price for France (gross)
  • ISBN 978-1-0716-0474-8
  • with online files
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Fasciola hepatica
Book Subtitle
Methods and Protocols
Editors
  • Martin Cancela
  • Gabriela Maggioli
Series Title
Methods in Molecular Biology
Series Volume
2137
Copyright
2020
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-0716-0475-5
DOI
10.1007/978-1-0716-0475-5
Hardcover ISBN
978-1-0716-0474-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 235
Number of Illustrations
13 b/w illustrations, 22 illustrations in colour
Topics

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