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Circadian Clocks

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Steven A. Brown

    1. Chronobiology and Sleep Research Group, Institute of Pharmacology and Toxicology, University of Zurich, Switzerland

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2130)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xxii
    Download chapter PDF

  2. Measuring and Modeling Physiological and Behavioral Rhythms

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Real-Time In Vitro Fluorescence Anisotropy of the Cyanobacterial Circadian Clock

      • Joel Heisler, Archana Chavan, Yong-Gang Chang, Andy LiWang
      Pages 3-18

    3. Inductively Coupled Plasma Mass Spectrometry for Elemental Analysis in Circadian Biology

      • Alessandra Stangherlin, Jason Day, John O’Neill
      Pages 19-27

    4. The Assessment of Circadian Rhythms Within the Immune System

      • Chloé C. Nobis, Marc Cuesta, Jean-François Daudelin, Geneviève Dubeau Laramée, Diane B. Boivin, Nathalie Labrecque et al.
      Pages 29-51

    5. Measuring Circadian Rhythms in Human Cells

      • Ngoc-Hien Du, Steven A. Brown
      Pages 53-67

    6. Measuring the Effects of Circadian Rhythm-Related Manipulations on Depression-Like Behavior in Rodents: Forced Swim and Tail Suspension Tests

      • Chelsea A. Vadnie, Lauren M. DePoy, Colleen A. McClung
      Pages 69-78

    7. Asking the Clock: How to Use Information from Questionnaires for Circadian Phenotyping

      • Céline Vetter, Eva C. Winnebeck, Till Roenneberg
      Pages 79-85

    8. Simple Kinetic Models in Molecular Chronobiology

      • J. Patrick Pett, Pål O. Westermark, Hanspeter Herzel
      Pages 87-100

  3. Genome-Wide Analyses in Circadian Biology

    1. Front Matter

      Pages 101-101
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    2. Searching Novel Clock Genes Using RNAi-Based Screening

      • Bert Maier, Stephan Lorenzen, Anna-Marie Finger, Hanspeter Herzel, Achim Kramer
      Pages 103-114

    3. Chromatin Immunoprecipitation (ChIP) from Mouse Liver Nuclei

      • Sara S. Fonseca Costa, Jürgen A. Ripperger
      Pages 115-125

    4. Using GRO-Seq to Measure Circadian Transcription and Discover Circadian Enhancers

      • Bin Fang, Dongyin Guan, Mitchell A. Lazar
      Pages 127-148

    5. Circadian Metabolomics from Breath

      • Steven A. Brown, Pablo Sinues
      Pages 149-156

    6. A Lipidomics View of Circadian Biology

      • Rona Aviram, Chunyan Wang, Xianlin Han, Gad Asher
      Pages 157-168

    7. Circadian Lipidomics: Analysis of Lipid Metabolites Around the Clock

      • Ursula Loizides-Mangold, Volodymyr Petrenko, Charna Dibner
      Pages 169-183

    8. Phosphoproteome and Proteome Sample Preparation from Mouse Tissues for Circadian Analysis

      • Franziska Brüning, Sean J. Humphrey, Maria S. Robles
      Pages 185-193

    9. Circadian Phosphorylation of CLOCK and BMAL1

      • Hikari Yoshitane, Yoshitaka Fukada
      Pages 195-203

  4. Imaging and Manipulating Brain Clocks

    1. Front Matter

      Pages 205-205
      Download chapter PDF

    2. Fluorescence Live Imaging of Drosophila Circadian Pacemaker Neurons

      • Virginie Sabado, Emi Nagoshi
      Pages 207-219
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Keywords

About this book

This volume presents techniques used by researchers from all branches of biology to study daily changes at a molecular level in many physiological systems. The chapters are organized into three parts and cover topics such as measuring and modeling physiological and behavioral rhythms; genome-wide analyses in circadian biology, and imaging and manipulating brain clocks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Circadian Clocks: Methods and Protocols is a valuable tool for any researcher interested in learning more about this developing field.



Editors and Affiliations

  • Chronobiology and Sleep Research Group, Institute of Pharmacology and Toxicology, University of Zurich, Switzerland

    Steven A. Brown

Bibliographic Information

  • Book Title: Circadian Clocks

  • Book Subtitle: Methods and Protocols

  • Editors: Steven A. Brown

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0381-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0380-2Published: 08 December 2020

  • Softcover ISBN: 978-1-0716-0383-3Published: 09 December 2021

  • eBook ISBN: 978-1-0716-0381-9Published: 07 December 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XXII, 342

  • Number of Illustrations: 11 b/w illustrations, 53 illustrations in colour

  • Topics: Biochemistry, general, Animal Models

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