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DNA Electrophoresis

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2119)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-x
  2. Two-Dimensional Gel Electrophoresis to Resolve DNA Topoisomers

    • Elizabeth G. Gibson, Alexandria A. Oviatt, Neil Osheroff
    Pages 15-24
  3. Analysis of DNA Interstrand Cross-Links and their Repair by Modified Comet Assay

    • Lonnie P. Swift, Lianne Castle, Peter J. McHugh
    Pages 79-88
  4. Analysis of Chromosomal DNA Fragmentation in Apoptosis by Pulsed-Field Gel Electrophoresis

    • Takeshi Terabayashi, Asako Tokumaru, Toshimasa Ishizaki, Katsuhiro Hanada
    Pages 89-99
  5. Pulsed-Field Gel Electrophoresis for Detecting Chromosomal DNA Breakage in Fission Yeast

    • Takatomi Yamada, Hiroshi Murakami, Kunihiro Ohta
    Pages 135-143
  6. Chromatin Pull-Down Methodology Based on DNA Triple Helix Formation

    • Asako Isogawa, Robert P. Fuchs, Shingo Fujii
    Pages 183-199
  7. Back Matter

    Pages 227-228

About this book

This volume explores the latest techniques used by researchers to study DNA electrophoresis, with focus on various species including bacteria, yeasts, and mammalian cells. The chapters in this book cover topics such as two-dimensional gel electrophoresis; DNA replication; pulsed-field gel electrophoresis; ChIP; and post-labeling/PAGE method for detection of DNA adducts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, DNA Electrophoresis: Methods and Protocols is a valuable resource for any researchers looking to learn more about this developing field.



Editors and Affiliations

  • Clinical Engineering Research Center, Faculty of Medicine, Oita University, Yufu, Japan

    Katsuhiro Hanada

Bibliographic Information

  • Book Title: DNA Electrophoresis

  • Book Subtitle: Methods and Protocols

  • Editors: Katsuhiro Hanada

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0323-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0322-2Published: 28 January 2020

  • Softcover ISBN: 978-1-0716-0325-3Published: 28 January 2021

  • eBook ISBN: 978-1-0716-0323-9Published: 27 January 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 228

  • Number of Illustrations: 30 b/w illustrations, 40 illustrations in colour

  • Topics: Human Genetics, Biomedical Engineering and Bioengineering

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access