Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2113)
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Table of contents (22 protocols)
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Front Matter
About this book
This volume looks at the different spectroscopic and biophysical methods used by researchers to study the structure and folding of RNA, and to follow their interactions with proteins. The chapters in this book cover topics such as single-molecule spectroscopy of multiple RNA species; surface plasmon resonance, MS or microcalorimetry for investigating molecular interactions with RNA; FTIR, SAXS, SANS and SRCD spectroscopies to analyze RNA structure; use of fluorescent nucleotides to map RNA-binding sites on proteins surfaces or CryoEM; and much more. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, RNA Spectroscopy: Methods and Protocols is a valuable resource for anyone interested in learning more about this developing field.
Editors and Affiliations
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Université de Paris, Paris, France, Laboratoire Léon Brillouin LLB, CEA, CNRS UMR12, Université Paris Saclay, Gif-sur-Yvette, France
Véronique Arluison
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Synchrotron SOLEIL L’Orme des Merisiers Saint Aubin, Gif-sur-Yvette, France
Frank Wien
Bibliographic Information
Book Title: RNA Spectroscopy
Book Subtitle: Methods and Protocols
Editors: Véronique Arluison, Frank Wien
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-0278-2
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020
Hardcover ISBN: 978-1-0716-0277-5Published: 01 February 2020
Softcover ISBN: 978-1-0716-0280-5Published: 01 February 2021
eBook ISBN: 978-1-0716-0278-2Published: 31 January 2020
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 356
Number of Illustrations: 16 b/w illustrations, 80 illustrations in colour
Topics: Human Genetics, Spectroscopy/Spectrometry