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Retinal Development

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2092)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
  2. Investigating Neurogenesis in Birds

    • Tania Rodrigues, Laurent Brodier, Jean-Marc Matter
    Pages 1-18
  3. Studying In Vivo Retinal Progenitor Cell Proliferation in Xenopus laevis

    • Cindy X. Kha, Dylan J. Guerin, Kelly Ai-Sun Tseng
    Pages 19-33
  4. Three-Dimensional Culture of Mouse Eyecups

    • Raven Diacou, Punita Bhansali, Wei Liu
    Pages 35-43
  5. Live Imaging of Mouse Retinal Slices

    • Anthony P. Barrasso, Ross A. Poché
    Pages 45-53
  6. Single-Cell Capture, RNA-seq, and Transcriptome Analysis from the Neural Retina

    • Rachayata Dharmat, Sangbae Kim, Yumei Li, Rui Chen
    Pages 159-186
  7. Paired Recording to Study Electrical Coupling Between Photoreceptors in Mouse Retina

    • Nange Jin, Zhijing Zhang, Kimberly A. Mankiewicz, Christophe P. Ribelayga
    Pages 221-230
  8. Back Matter

    Pages 231-235

About this book

This volume details commonly used molecular and cellular techniques and specialized methodologies for studying retina neuronal subtypes and electrophysiology. Chapters describe techniques for anatomical studies of retinal ganglion cell morphology, gap-junction-mediated neuronal connection, multi-electrode array recording on mouse retinas, and paired recording to study the electrical coupling between photoreceptors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Retinal Development: Methods and Protocols aims to provide readers with a set of practical experimental tools to study retinal development, regeneration, and function of mature retinal neurons. Many of the protocols and strategies described in one organism can be easily adapted to applications in different model systems.

Editors and Affiliations

  • Ruiz Department of Ophthalmology and Visual Science, McGovern Medical School at The University of Texas Health Science Center at Houston (UTHealth), Houston, USA

    Chai-An Mao

Bibliographic Information

  • Book Title: Retinal Development

  • Book Subtitle: Methods and Protocols

  • Editors: Chai-An Mao

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0175-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0174-7Published: 01 December 2019

  • Softcover ISBN: 978-1-0716-0177-8Published: 18 January 2021

  • eBook ISBN: 978-1-0716-0175-4Published: 30 November 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 235

  • Number of Illustrations: 11 b/w illustrations, 48 illustrations in colour

  • Topics: Developmental Biology, Neurosciences

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access