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Nucleic Acid Detection and Structural Investigations

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Kira Astakhova

    1. Department of Chemistry, Technical University of Denmark, Kongens Lyngby, Denmark

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Syeda Atia Bukhari

    1. Department of Chemistry, Technical University of Denmark, Kongens Lyngby, Denmark

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2063)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. In Vitro Detection

    1. Front Matter

      Pages 1-1
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    2. Optomagnetic Detection of Rolling Circle Amplification Products

      • Gabriel Antonio S. Minero, Valentina Cangiano, Jeppe Fock, Francesca Garbarino, Mikkel F. Hansen
      Pages 3-15

    3. Subtyping of Swine Influenza Using a High-Throughput Real-Time PCR Platform and a Single Microfluidics Device

      • Helene Larsen, Nicole B. Goecke, Charlotte K. Hjulsager
      Pages 17-25

    4. RT-qPCR Detection of Low-Copy HIV RNA with Yin-Yang Probes

      • Dmitry E. Kireev, Valentina M. Farzan, German A. Shipulin, Vladimir A. Korshun, Timofei S. Zatsepin
      Pages 27-35

    5. Solid-Phase Hybridization Assay for Detection of Mutated Cancer DNA by Fluorescence

      • Maria Taskova, Kira Astakhova
      Pages 37-44

    6. 5′-Monopyrene and 5′-Bispyrene 2′-O-methyl RNA Probes for Detection of RNA Mismatches

      • D. S. Novopashina, O. A. Semikolenova, A. G. Venyaminova
      Pages 45-56

    7. Combined Assay for Detecting Autoantibodies to Nucleic Acids and Apolipoprotein H in Patients with Systemic Lupus Erythematosus

      • Sangita Khatri, Elizabeth D. Mellins, Kathryn S. Torok, Syeda Atia Bukhari, Kira Astakhova
      Pages 57-71

    8. Electrochemical Detection of dsDNA-Specific Antibodies

      • Pablo Fagúndez, Gustavo Brañas, Justo Laíz, Juan Pablo Tosar
      Pages 73-83

  3. Nanotechnology and Imaging

    1. Front Matter

      Pages 85-85
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    2. Sequence-Defined DNA Amphiphiles for Drug Delivery: Synthesis and Self-Assembly

      • Michael D. Dore, Hanadi F. Sleiman
      Pages 87-100

    3. DNA-Mediated Liposome Fusion Observed by Fluorescence Spectrometry

      • Philipp M. G. Löffler, Oliver Ries, Stefan Vogel
      Pages 101-118

    4. Advanced Fluorescence Imaging to Distinguish Between Intracellular Fractions of Antisense Oligonucleotides

      • M. Leontien van der Bent, Derick G. Wansink, Roland Brock
      Pages 119-138

    5. Methodology for Subcellular Fractionation and MicroRNA Examination of Mitochondria, Mitochondria Associated ER Membrane (MAM), ER, and Cytosol from Human Brain

      • Paresh Prajapati, Wang-Xia Wang, Peter T. Nelson, Joe E. Springer
      Pages 139-154

  4. Biomedical Applications and Big Data

    1. Front Matter

      Pages 155-155
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    2. Skeletal Muscle Injury by Electroporation: A Model to Study Degeneration/Regeneration Pathways in Muscle

      • Camila F. Almeida, Mariz Vainzof
      Pages 157-169

    3. Isolation and Characterization of Muscle-Derived Stem Cells from Dystrophic Mouse Models

      • Paula C. G. Onofre-Oliveira, Mariz Vainzof
      Pages 171-180

    4. Universal Library Preparation Protocol for Efficient High-Throughput Sequencing of Double-Stranded RNA Viruses

      • Anna S. Dolgova, Marina V. Safonova, Vladimir G. Dedkov
      Pages 181-188

    5. Quantitation of Molecular Pathway Activation Using RNA Sequencing Data

      • Nicolas Borisov, Maxim Sorokin, Andrew Garazha, Anton Buzdin
      Pages 189-206

    6. Molecular Pathway Analysis of Mutation Data for Biomarkers Discovery and Scoring of Target Cancer Drugs

      • Marianna Zolotovskaia, Maxim Sorokin, Andrew Garazha, Nikolay Borisov, Anton Buzdin
      Pages 207-234
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Keywords

About this book

This book volume provides in depth coverage of the nucleic acid field and aims to represent a broad diversity of the methodologies and a wide range of nucleic-acid-related topics within the fields of molecular biology and biomedicine. Chapters are divided in three sections detailing in vitro detection, nanotechnology and imaging, biomedical applications, and big data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Nucleic Acid Detection and Structural Investigations: Methods and Protocols aims present aims to present methodologies combine cutting-edge innovation with sound theory and practical applications in life sciences.


Editors and Affiliations

  • Department of Chemistry, Technical University of Denmark, Kongens Lyngby, Denmark

    Kira Astakhova, Syeda Atia Bukhari

Bibliographic Information

  • Book Title: Nucleic Acid Detection and Structural Investigations

  • Book Subtitle: Methods and Protocols

  • Editors: Kira Astakhova, Syeda Atia Bukhari

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0138-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0137-2Published: 31 October 2019

  • Softcover ISBN: 978-1-0716-0140-2Published: 31 October 2020

  • eBook ISBN: 978-1-0716-0138-9Published: 30 October 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 270

  • Number of Illustrations: 24 b/w illustrations, 48 illustrations in colour

  • Topics: Biochemistry, general

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