CRISPR-Cas applied to TGF-beta induced EMT © LABSTER

CRISPR-Cas applied to TGF-beta induced EMT

CRISPR may sound like a brand of cereals, but it certainly isn’t! In this simulation, you will learn to identify the molecular hallmarks of the Epithelial-to-Mesenchymal Transition (EMT) molecular process in breast cancer cells. You will induce EMT by adding TGF-beta to the cells, and, after identifying the hallmarks with immunofluorescence labeling, you will use the cutting-edge CRISPR-Cas9 technology to try to revert this process, which is one of the main causes of cancer malignancy.

At the end of this simulation, you will be able to…

• Set up a protocol to get answers to a biological question related to the EMT process

• Acknowledge mistakes in the protocols and understand how to critically overcome them

• Properly prepare cells for the immunostaining procedure according to the localization of the target protein

• Select a suitable secondary antibody for an immunofluorescence experiment

• Analyze immunofluorescence results

• Describe the basics of CRISPR-Cas technique

• Design a guide RNA construct for knock-out strategies

• Evaluate CRISPR-Cas9 results

Techniques in Lab: Immunofluorescence | CRISPR-Cas9

Contact | Information material

For further information about our in-house seminars, company terms and conditions or questions regarding our content please get in touch with us via email, phone or the contact form. We would also be pleased to send you our 'Offers for companies' information brochure. We look forward to hearing from you.

Merlet Behncke-Braunbeck
Director Distance Learning
Tel.:  +49 (0) 6221 487 8061

Please contact me directly or use the quick & easy
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Date | Price | Audience


Virtual Lab Simulation

Co-operation partner: 


Time to complete course: 

30 min




please contact Merlet Behncke-Braunbeck


Start possible at any time


Companies can book this course for their employees directly at Springer Campus.

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