CRISPR-Cas applied to TGF-beta induced EMT
CRISPR may sound like a brand of cereals, but it certainly isn’t! In this simulation, you will learn to identify the molecular hallmarks of the Epithelial-to-Mesenchymal Transition (EMT) molecular process in breast cancer cells. You will induce EMT by adding TGF-beta to the cells, and, after identifying the hallmarks with immunofluorescence labeling, you will use the cutting-edge CRISPR-Cas9 technology to try to revert this process, which is one of the main causes of cancer malignancy.
At the end of this simulation, you will be able to…
• Set up a protocol to get answers to a biological question related to the EMT process
• Acknowledge mistakes in the protocols and understand how to critically overcome them
• Properly prepare cells for the immunostaining procedure according to the localization of the target protein
• Select a suitable secondary antibody for an immunofluorescence experiment
• Analyze immunofluorescence results
• Describe the basics of CRISPR-Cas technique
• Design a guide RNA construct for knock-out strategies
• Evaluate CRISPR-Cas9 results
Techniques in Lab: Immunofluorescence | CRISPR-Cas9
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Director Distance Learning
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Date | Price | Audience
Virtual Lab Simulation
Time to complete course:
please contact Merlet Behncke-Braunbeck
Start possible at any time
Companies can book this course for their employees directly at Springer Campus.