With this package you will soon master the techniques needed to work in a genetics lab! The package offers you the following 5 simulations:
Learn the principles of Mendelian inheritance. In this simulation you will also learn what inheritance looks like at the molecular level.
Polymerase Chain Reaction
Learn the techniques and application of Polymerase Chain Reaction and Gel Electrophoresis. Explore real world application, such as analyzing unique genetic fingerprints to solve a murder case.
Learn about the Mendelian inheritance of double muscling in cattle. Find the responsible gene using linkage analysis and learn how mutation in this gene alters gene expression resulting in double muscled cattle.
Discover Next Generation Sequencing by analyzing the mRNA from pig tissues and identify a new gene linked to obesity. Confirm your results using qPCR to analyze the expression levels of your gene!
Open your eyes to the vast potential of gene regulation, where you can reprogram cells into any cell that fits your needs. Will you able to help the doctor in restoring the sight of a visually impaired girl?
About the Mendelian Inheritance Virtual Simulation Lab
In the Mendelian Inheritance simulation you will learn the basic principles of Mendelian inheritance. You will study how Mendel’s postulates can be applied to determine how characteristics are passed from one generation to the next. You will also learn what inheritance looks like at the molecular level. Can you find out if your patient’s children will inherit his color blindness?
Cross purebred mice and observe their phenotypes
To understand the basic laws of inheritance, you will perform mouse experiments and observe how a mouse’s genes can influence its fur color. Can you figure out which fur color is dominant?
Predict the genotypes in the patient’s family
Instead of performing experiments, you will discover how Punnett squares and pedigree trees can be used to predict the genetic makeup of a family. What is true for the fur color in mice, can also be applied to human color blindness!
Experience inheritance at the cellular level
Dive deeper into the laws of inheritance as you watch how cells divide to become gametes inside the reproductive system. By unraveling the laws of X-linked inheritance discover why color blindness affects more men than women.
About the Polymerase Chain Reaction Virtual Simulation Lab:
In the Polymerase Chain Reaction (PCR) simulation you will be thrown right into a crime scene where a murder has taken place. To investigate the crime scene your first task is to collect blood samples in the hope that the murderer has left traces of their DNA.
After sampling you will go to the lab to isolate and analyze the sample of DNA you collected. By using a PCR kit, a thermocycler, and the purified DNA from the crime scene, it is up to you to mix the correct reagents and perform the PCR experiment.
See the structure of DNA and its replication up close
A 3D animation will show the PCR experiment at the molecular level, illustrating the structure of DNA and its replication. Quiz questions will be asked throughout the experimental process, as well as at specific steps of the PCR itself.
Identify the murderer
In the PCR simulation, you will your collected sample and other prepared samples from the suspects on a gel, and then compare the patterns that emerge.
Will you be able to identify the murderer?
About the Animal Genetics Virtual Simulation Lab:
In the Animal Genetics simulation you will learn about Mendelian inheritance and how a mutation in DNA can give rise to an altered phenotype. You will use polymerase chain reaction (PCR) and gel electrophoresis to perform genotyping in order to identify the gene that causes double muscling in cattle.
Fill in the pedigree
As a researcher you will be called to a farm to investigate a case about double muscled cattle. In your first task you will fill in a pedigree to help identify the double muscled cattle. By studying the pedigree, you will learn about heritable traits and whether double muscling is autosomal or sex-linked and whether it is dominant or recessive. You will then draw a blood sample, and from that, extract DNA and perform further experiments in the lab.
Identify the candidate gene
Your next task will be to perform genome scanning, which involves an analysis of the entire genome and identifying the candidate gene by using short tandem repeats (STRs) as markers. By detecting STR patterns and similar gene sequences in double muscled cattle DNA, your goal will be to narrow down the genomic location of the candidate gene. Experimentally, this will be achieved by amplifying the STRs using PCR and analyzing the PCR product size using gel electrophoresis.
Develop a DNA test
After identifying the gene responsible for double muscling, your last task will be to develop a DNA test. This test will be used to determine the origin of organic meat; as it is prohibited to label meat as organic if derived from double muscled cattle. You will use your newly developed DNA test on three meat packages from an organic farm.
Will you be able to use your understanding of animal genetics to find the gene which causes double muscling and develop a reliable commercial test for organic meat?
About the Gene Expression Unit Virtual Simulation Lab:
It is now well-known that obesity is linked to genes in your DNA – but which ones are they? In this simulation, you will learn how to sequence DNA and screen for genes that might be over-expressed in obese pigs. To do so, you will have to use state-of-the-art techniques such as Next Generation Sequencing and qPCR and perform proper data analysis to single out specific genes.
Identify a new gene linked to obesity
As part of a project to study obesity and using pig samples as a model, students will sequence the messenger RNA molecules to identify if a gene is overexpressed in an obese pig compared to a lean one. They will confirm their results with a different technique, quantitative PCR (qPCR) and learn the role of the gene in the human metabolism.
Next Generation Sequencing and qPCR
In order to sequence their samples, the student will first have to perform a RT-PCR to generate cDNA from mRNA, then prepare the samples for Next Generation Sequencing by adding the proper genetic labels. After sequencing, they will analyze their data to single out a gene that could be linked to obesity. To confirm their findings, the students will also have to design a quantitative PCR (qPCR) with the proper controls and analyze the resulting curves. This simulation allows students to see the process inside the Next Generation Sequencing machine, as well as perform two high-level techniques (sequencing and qPCR) in a very short period of time.
Publish your data
The discovery of a new gene which could partially explain the effect of obesity would be worth publishing in an eminent scientific journal. Will you be able to single out such a gene from your sequencing experiment?
About the Gene Regulation Virtual Simulation Lab:
In the past, there was no hope for visually impaired people to regain their sight back. Welcome to the future of medicine! In this simulation, you will learn the gene regulation principle that won the Nobel Prize for Physiology 2012. With the gene regulation technique develop by Sir Gurdon and Shinya Yamanaka, your mission is to try to treat a visually impaired girl.
Learn the basics of stem cells
In order to save the visually impaired girl, you will prepare induced pluripotent stem cells (iPS) from a fibroblast cells sample taken from the girl. The iPS cells will then be differentiated to retinal-pigmented epithelial cells for transplantation. In this simulation, 3D-animations will allow you to take a closer look at the machinery involved in gene regulation. You will learn about the basics of stem cells and what it takes to create one.
Transform fibroblast cells into iPS cells
You will have to determine which transcription factors are essential in maintaining a stem cell’s features based on what you have learned in the previous mission. After determining the genes, you will use a retroviral infection technique to transform mature fibroblast into iPS cells. After incubation, you will examine which set of transcription factors is capable of inducing the fibroblast into stem cells (iPS).
Analyze gene expression
By now you will have an idea of which transcription factor is capable of transforming fibroblast cells into iPS cells. The next step is to confirm your findings by analyzing the gene expression in mRNA and protein level. You will perform reverse transcription PCR (RT-PCR) and Western blot to investigate the mRNA and protein level, respectively. Finally, you will have to decide which set of transcription factors can be used to transform fibroblast cells into iPS effectively. Will you be able to help the girl?
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Date | Price | Audience
Package: 5 courses
Time to complete course:
30 - 50 min
please contact Merlet Behncke-Braunbeck
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Companies can book this course for their employees directly at Springer Campus.