Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT), Dublin, Ireland, June 7-10, 2009

Synthetic and Molecular Biology

1. Front Matter

   Pages 1-1

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2. MicroRNAs as Potential Engineering Targets for Improvement of CHO Cell Production Phenotypes

   • Niall Barron, Niraj Kumar, Noelia Sanchez, Patrick Gammell, Martin Clynes

   Pages 3-11

3. Are Clones Really Unstable?

   • Kriti Shukla, Saravanan Desan, Ankur Bhatnagar, Anuj Goel

   Pages 13-18

4. An Optimised Transfection Platform for the Epi-CHO Transient Expression System in Serum-free Media

   • Joe Codamo, Trent P. Munro, Benjamin S. Hughes, Michael Song, Peter P. Gray

   Pages 19-23

5. Using the “OMICS” Technologies as Complementary Tools to Study the Molecular Mechanisms Involved with the Adaptation of Myeloma Cell Line to Protein-Free Medium

   • K.R. de la Luz-Hernández, Y. Rabasa-Legón, A. Lage-Castellanos, A. Castillo-Vitlloch, L. Castellanos-Serra, J. Díaz-Brito et al.

   Pages 25-29

6. Galectin-3 Over-Expression Enhances Survival and Recombinant Protein Expression in Mammalian Cells

   • Fanny Delegrange, Mattia Matasci, Lucia Baldi, Florian M. Wurm

   Pages 31-35

7. Metabolomic Analysis of CHO Cultures with Different Growth Characteristics – Development of a Metabolite Extraction Protocol for Suspension Adapted Mammalian Cells

   • Stefanie Dietmair, Nicholas E. Timmins, Panagiotis Chrysanthopoulos, Peter P. Gray, Jens O. Krömer, Lars K. Nielsen

   Pages 37-41

8. Cell Lines in Four Weeks with the CEMAX® System

   • Benedikt Greulich, Karlheinz Landauer, Andreas Herrmann

   Pages 43-46

9. Analysis of Protein Expression via Alternate 3’ Untranslated Region (UTR) Signals Through the Use of Site Specific Recombination

   • Jeff Jia Cheng Hou, Michael Song, Trent P. Munro, Peter P. Gray

   Pages 47-51

10. A Case Study in Clone Screening: A Comprehensive Approach for a Product With High Projected Market Demand

    • Kirin M. Jamison, Dacia R. Brooks, Szu-Han Wang, Martin Gawlitzek

    Pages 53-56

11. An Improved Clone Selection Method

    • Uros Jamnikar, Marjanca Blas, Dominik Gaser, Jerica Rozman-Pungercar, Andrej Francky

    Pages 57-60

12. Dissecting the Mechanism of Action of BHRF1 for the Protection Against Apoptosis in MAb-Producing Cell Lines

    • Ernest Milián, Sandra Juanola, Eva Prats, Jordi J. Cairó, Francesc Gòdia, Joaquim Vives

    Pages 61-65

13. Qualification of Bench and Pilot-Scale Bioreactors as Models of Commercial Systems

    • Anne Marie Molloy, Patrick Dowling, Mairead Looby, Caitriona Crawford, Bruce Tangarone, Mary Heenan et al.

    Pages 67-70

14. Quantification of Polyethylenimine in Transient Gene Expression: On the Way to GMP Compliance

    • Sophie Nallet, Zuzana Kadlecova, Lucia Baldi, Harm-Anton Klok, Florian M. Wurm

    Pages 71-75

15. Implications of Vector Fragmentation and Initial Production Levels in Production Cell Line Development Using MTX

    • Say Kong Ng, Wenyu Lin, Rohit Sachdeva, Daniel I.C. Wang, Miranda G.S. Yap

    Pages 77-83

16. Characterizing the Expression Stability in Different Phenotypes of Recombinant NS0 Myeloma Cell Lines

    • Y. Prieto, L. Rojas, L. Hinojosa, K.R. de la Luz-Hernández, D. Aguiar, S. Victores et al.

    Pages 85-90

17. A Transient Gene Expression Process with Recombinant Antibody Titer of 0.5 g/L in CHO Cells

    • Yashas Rajendra, Gaurav Backliwal, Markus Hildinger, Sarah Wulhfard, Maria de Jesus, Florian M. Wurm

    Pages 91-94

18. Proteomic Profiling of Temperature-Shifted CHO Cells to Identify Genes that Impact on Both Cell Growth and Recombinant Protein Productivity in Suspension Culture

    • Niraj Kumar, Noelia Sanchez, Patrick Gammell, Patrick Dowling, M. Henry, P. Meleady et al.

    Pages 95-107

19. Stabilization of Protein Expression in Mammalian Cells Employing a Toxin/Antitoxin Based Strategy

    • Kristina Nehlsen, Jeannette Zauers, Sabrina Herrmann, Hansjörg Hauser, Dagmar Wirth

    Pages 109-113