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Restriction Landmark Genomic Scanning (RLGS)

  • Book
  • © 1997

Overview

Editors:
  1. Yoshihide Hayashizaki

    1. Genome Science Laboratory, RIKEN, Tsukuba Ibaraki, Japan

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  2. Sachihiko Watanabe

    1. Shionogi Institute for Medical Science, Settsu, Osaka, Japan

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    You can also search for this editor in PubMed   Google Scholar

  • The first laboratory manual with detailed techniques on RLGS, a rapid method for genome-wide scanning with broad applications in biological and medical research.

Part of the book series: Springer Lab Manuals (SLM)

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Table of contents (9 chapters)

  1. Front Matter

    Pages I-XII
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  2. Concept of Genome Scanning

    • Yoshihide Hayashizaki
    Pages 1-7

  3. Principle of RLGS

    • Yoshihide Hayashizaki
    Pages 9-16

  4. Protocols for RLGS Gel Production

    • Yasushi Okazaki, Hisato Okuizumi, Nobuya Sasaki, Shuji Takada, Tokuei Takahara, Yoshihide Hayashizaki
    Pages 17-36

  5. RLGS Spot Cloning

    • Sachihiko Watanabe, Yoshihide Hayashizaki
    Pages 37-56

  6. RLGS Spot Mapping Method

    • Hisato Okuizumi, Yasushi Okazaki, Yoshihide Hayashizaki
    Pages 57-93

  7. Application of RLGS to Screening Endogeneously Imprinted Genes

    • Hideo Shibata, Christoph Plass
    Pages 95-114

  8. Systematic Detection of DNA Alteration in Cancer Tissue

    • Yasushi Okazaki, Tomoya Ohsumi, Hisato Okuizumi, William A. Held, Yoshihide Hayashizaki
    Pages 115-128

  9. RLCS, Restriction Landmark cDNA Scanning

    • Takeshi Yaoi, Harukazu Suzuki, Jun Kawai, Sachihiko Watanabe
    Pages 129-156

  10. Concluding Remarks

    • Yoshihide Hayashizaki
    Pages 157-158

  11. Back Matter

    Pages 159-179
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Keywords

About this book

Restriction Landmark Genomic Scanning (RLGS) is a new multiplex method for simultaneous analysis of over 3,000 genome loci. Written by the inventor of RLGS, Yoshihide Hayashizaki, and his co-workers, this is the first manual on this method. RLGS is a powerful technique with enormous potential for biological (animal and plant sciences), and biomedical research. Yielding results much faster than conventional techniques, RLGS is particularly suited to the identification of the chromosomal location of the genes implicated in inherited diseases, and for the genetic disturbances present in cancerous tissue.

Editors and Affiliations

  • Genome Science Laboratory, RIKEN, Tsukuba Ibaraki, Japan

    Yoshihide Hayashizaki

  • Shionogi Institute for Medical Science, Settsu, Osaka, Japan

    Sachihiko Watanabe

Bibliographic Information

  • Book Title: Restriction Landmark Genomic Scanning (RLGS)

  • Editors: Yoshihide Hayashizaki, Sachihiko Watanabe

  • Series Title: Springer Lab Manuals

  • DOI: https://doi.org/10.1007/978-4-431-67953-0

  • Publisher: Springer Tokyo

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer Japan 1997

  • Softcover ISBN: 978-4-431-68521-0Published: 13 November 2013

  • eBook ISBN: 978-4-431-67953-0Published: 29 June 2013

  • Edition Number: 1

  • Number of Pages: XII, 179

  • Topics: Biochemistry, general, Cell Biology, Molecular Medicine

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