Skip to main content
SpringerLink
Log in

Basic Cloning Procedures

  • Book
  • © 1998

Overview

Editors:
  1. Valdis Berzins

    1. Biomedical Research and Study Centre, University of Latvia, Riga, Latvia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Basic laboratory techniques, approved in several international molecular biology courses
  • Step-by-step protocols with detailed practical hints, troubleshooting guide and short background information
  • The protocols are written by authors with intensive practical experience

Part of the book series: Springer Lab Manuals (SLM)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 39.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (8 chapters)

  1. Front Matter

    Pages i-vii
    Download chapter PDF

  2. cDNA Synthesis and Cloning

    • Juris Steinbergs, Aleksander Tsimanis
    Pages 1-21

  3. DNA Sequencing

    • Eriks Jankevics
    Pages 22-42

  4. RNA In Vitro Synthesis by Phage T7 DNA-Dependent RNA Polymerase

    • Elita Avota, Normunds Licis
    Pages 43-52

  5. PCR-Based Site-Specific Mutagenesis

    • Janis Klovins, Valdis Berzins
    Pages 53-67

  6. Analysis of Specific Protein-DNA Interactions

    • Indrikis Muiznieks, Nils Rostoks
    Pages 68-103

  7. Gel Electrophoresis, Transfer and Hybridization of Nucleic Acids

    • Illar Pata, Erkki Truve
    Pages 104-134

  8. Immunological Methods for Analysis of Recombinant Proteins

    • Irina Sominskaya, Kaspars Tars
    Pages 135-144

  9. Protein Synthesis in Cell-Free Systems

    • Tiina Tamm, Erkki Truve
    Pages 145-163

  10. Back Matter

    Pages 165-169
    Download chapter PDF
Back to top

Keywords

About this book

This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis. Each protocol includes short background information, a detailed description of the necessary materials, step-by-step procedures, a troubleshooting guide and useful practical hints.

Editors and Affiliations

  • Biomedical Research and Study Centre, University of Latvia, Riga, Latvia

    Valdis Berzins

Bibliographic Information

  • Book Title: Basic Cloning Procedures

  • Editors: Valdis Berzins

  • Series Title: Springer Lab Manuals

  • DOI: https://doi.org/10.1007/978-3-642-71965-3

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 1998

  • Softcover ISBN: 978-3-642-48977-8Published: 07 July 2012

  • eBook ISBN: 978-3-642-71965-3Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: VII, 169

  • Topics: Biochemistry, general, Cell Biology

Publish with us

Policies and ethics

Back to top

Search

Navigation