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Protein Structure Analysis

Preparation, Characterization, and Microsequencing

  • Book
  • © 1997

Overview

Editors:
  1. Roza Maria Kamp

    1. Technische Fachhochschule Berlin FB3 Chemie- und Biotechnik, Berlin, Germany

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  2. Theodora Choli-Papadopoulou

    1. School of Biochemistry, Laboratory of Biochemistry, Aristotle University Thessaloniki, Thessaloniki, Greece

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  3. Brigitte Wittmann-Liebold

    1. Max-Delbrück-Centrum für Molekulare Medizin, Berlin-Buch, Germany

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Part of the book series: Springer Lab Manuals (SLM)

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Table of contents (21 chapters)

  1. Front Matter

    Pages I-XIII
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  3. Separation of Peptides

    1. Front Matter

      Pages 59-59
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    2. Enzymatic and Chemical Cleavages of Proteins

      • R. Kraft
      Pages 61-71

    3. Separation of Peptides Using HPLC and TLC Fingerprints

      • T. Choli-Papadopoulou, R. M. Kamp
      Pages 73-83

    4. Microseparation of In Situ Digested Peptides

      • A. Otto
      Pages 85-95

    5. Isolation of Peptides for Microsequencing by In-Gel Proteolytic Digestion

      • U. Hellman
      Pages 97-104

  4. Manual and Automated Microsequencing Methods

    1. Front Matter

      Pages 105-105
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    2. Automated Microsequencing: Introduction and Overview

      • B. Wittmann-Liebold
      Pages 107-136

    3. A Manual Method for Protein Sequence Analysis Using the DABITC/PITC Method

      • T. Choli-Papadopoulou, Y. Skendros, K. Katsani
      Pages 137-151

    4. Solid-Phase Sequencing of Peptides and Proteins

      • J. Salnikow
      Pages 153-165

    5. Sequence Analysis of the NH2-Terminally Blocked Proteins Immobilized on PVDF Membranes from Polyacrylamide Gels

      • H. Hirano
      Pages 167-179

  5. Electrophoresis and Blotting of Proteins

    1. Front Matter

      Pages 181-181
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    2. Two-Dimensional Electrophoresis

      • P. Jungblut
      Pages 183-214

    3. Semi-Dry Blotting onto Hydrophobic Membranes

      • P. Jungblut
      Pages 215-227

  6. Analysis of Amino Acids

    1. Front Matter

      Pages 229-229
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Keywords

About this book

"Protein Structure Analysis - Preparation and Characterization" is a compilation of practical approaches to the structural analysis of proteins and peptides. Here, about 20 authors describe and comment on techniques for sensitive protein purification and analysis. These methods are used worldwide in biochemical and biotechnical research currently being carried out in pharmaceu­ tical and biomedical laboratories or protein sequencing facilities. The chapters have been written by scientists with extensive ex­ perience in these fields, and the practical parts are well documen­ ted so that the reader should be able to easily reproduce the described techniques. The methods compiled in this book were demonstrated in student courses and in the EMBO Practical Course on "Microsequence Analysis of Proteins" held in Berlin September 10-15, 1995. The topics also derived from a FEBS Workshop, held in Halkidiki, Thessaloniki, Greece, in April, 1995. Most of the authors participated in these courses as lecturers and tutors and made these courses extremely lively and successful. Since polypeptides greatly vary depending on their specific structure and function, strategies for their structural analysis must for the most part be adapted to each individual protein. Therefore, advantages and limitations of the experimen­ tal approaches are discussed here critically, so that the reader becomes familiar with problems that might be encountered.

Reviews

"The most advanced and sensitive methods have been selected for this manual. The presented procedures are well established and have been found useful in many laboratories."
Bioscope

Editors and Affiliations

  • Technische Fachhochschule Berlin FB3 Chemie- und Biotechnik, Berlin, Germany

    Roza Maria Kamp

  • School of Biochemistry, Laboratory of Biochemistry, Aristotle University Thessaloniki, Thessaloniki, Greece

    Theodora Choli-Papadopoulou

  • Max-Delbrück-Centrum für Molekulare Medizin, Berlin-Buch, Germany

    Brigitte Wittmann-Liebold

Bibliographic Information

  • Book Title: Protein Structure Analysis

  • Book Subtitle: Preparation, Characterization, and Microsequencing

  • Editors: Roza Maria Kamp, Theodora Choli-Papadopoulou, Brigitte Wittmann-Liebold

  • Series Title: Springer Lab Manuals

  • DOI: https://doi.org/10.1007/978-3-642-59219-5

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 1997

  • Softcover ISBN: 978-3-642-47765-2Published: 07 November 2012

  • eBook ISBN: 978-3-642-59219-5Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: XIII, 316

  • Topics: Biochemistry, general, Cell Biology

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