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Nucleic Acid Electrophoresis

  • Book
  • © 1998

Overview

Editors:
  1. Dietmar Tietz

    1. Laurel, USA

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  • Step-by-step protocols with materials, troubleshooting and suppliers sections for immediate use at the lab bench
  • All technical details needed to perform successful experiments are included Clear structure and presentation Protocols are written by experts in the relevant techniques

Part of the book series: Springer Lab Manuals (SLM)

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Table of contents (13 chapters)

  1. Front Matter

    Pages I-XIII
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  2. DNA Gel Electrophoresis

    • Nancy C. Stellwagen
    Pages 1-53

  3. Denaturating Electrophoresis in DNA Sequencing: A Brief History and Current Protocols

    • Alexander Kraev
    Pages 54-98

  4. An Introduction to DNA Fingerprinting Using RFLP and RAPD Techniques

    • R. J. Snowdon, A. Langsdorf
    Pages 99-128

  5. Pulsed-Field Gel Electrophoresis for Genomic Fingerprinting of Pathogenic Bacteria

    • Michael B. Coulthart, Wendy M. Johnson, Fraser E. Ashton
    Pages 129-151

  6. Ultrasensitive Silver Based Stains for Nucleic Acid Detection

    • Carl R. Merril, Karen M. Washart, Robert C. Allen
    Pages 152-161

  7. The Analysis of Microsatellites by Silver Staining of Nondenaturing Polyacrylamide Gels

    • Shirin S. Joseph
    Pages 162-169

  8. Capillary Electrophoretic Separation of DNA Fragments

    • John M. Butler, Dennis J. Reeder
    Pages 170-192

  9. Rapid Detection of Hepatitis C Virus in Plasma and Liver Biopsies by Capillary Electrophoresis

    • Sameer A. Sakallah, Robert W. Lanning, David L. Cooper
    Pages 193-201

  10. Electrophoresis in the Field of Forensic Science

    • Hildegard Haas-Rochholz
    Pages 202-238

  11. The Electrophoretic Mobility Shift Assay (EMSA) for Detection and Analysis of Protein-DNA Interactions

    • Michael G. Fried, Mark M. Garner
    Pages 239-271

  12. Mobility Shift Analysis of Protein-DNA Complexes by Capillary Electrophoresis

    • Jun Xian, Michael G. Harrington
    Pages 272-291

  13. Polyacrylamide Gel Electrophoresis of DNA/Protein Complexes

    • Andreas Kyas, Winfried Mäueler, Joerg T. Epplen
    Pages 292-310

  14. Detection of DNA Curvature Using Transverse Pore Gradient Polyacrylamide Gel Electrophoresis

    • David Wheeler
    Pages 311-324

  15. Back Matter

    Pages 325-328
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Keywords

About this book

Electrophoresis is a powerful method to analyze nucleic acids (DNA, RNA). Various sophisticated techniques such as capillary electrophoresis, pulsed-field electrophoresis, fingerprinting using RFLP and RAPD, DNA sequencing, and mobility shift assay are described in detail. The required apparatus, appropriate use, preparation of probes, gel staining, interpretation of results, tricks for troubleshooting, manufacturers addresses, helpful Internet resources as well as specific applications, e.g. in legal medicine, microbiology and agriculture, are presented by leading experts.

Editors and Affiliations

  • Laurel, USA

    Dietmar Tietz

Bibliographic Information

  • Book Title: Nucleic Acid Electrophoresis

  • Editors: Dietmar Tietz

  • Series Title: Springer Lab Manuals

  • DOI: https://doi.org/10.1007/978-3-642-58924-9

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 1998

  • Softcover ISBN: 978-3-642-47763-8Published: 07 November 2012

  • eBook ISBN: 978-3-642-58924-9Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: XIII, 328

  • Topics: Biochemistry, general, Biotechnology, Human Genetics

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